Microbial foliage synergist
A technology of microorganisms and synergists, applied in the direction of organic fertilizers, etc., can solve the problems of low utilization, insufficient technological innovation, unstable product quality, etc., and achieve the effect of increasing yield and ensuring stability.
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[0084] 1. Preparation of Bacillus lateralis:
[0085] a. Strain selection
[0086] (Ampoule tube) shape and arrangement: the fungus is rod-shaped, with a slightly thinner end, solitary in pairs and piles; the spores are oval, (1-1.2)×(2.5-3) μm, with canoes attached to the side of the spores Shape, spores grow laterally and mesolaterally in spindle-shaped cysts.
[0087] Movement and flagella: able to move.
[0088] b. Bacteria cultivation
[0089] Slant culture: test tube slant medium: beef extract-peptone medium; culture temperature: 32±1°C; culture time: 72-96 hours.
[0090] Shake flask culture: culture temperature: 32±1°C culture time: 30-32 hours
[0091] c. Formulation production
[0092] Seed tank liquid fermentation culture: culture temperature: 32±1°C culture time: 18-20 hours aeration condition 1: 0.3-0.5V / V
[0093] Closed solid fermentation incubator culture:
[0094] Cultivation temperature: 28-35°C Cultivation time: 96-120 hours Cultivation humidity: 60%-...
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