Western blot kit for specific IgG antibodies of syphilis and preparation method thereof

Abstract

The invention provides a western blot kit for specific IgG antibodies of syphilis and a preparation method thereof, relating to a kit. The kit is provided with a carrier plate, a cellulose nitrate membrane, a detection line of the specific IgG antibodies of the syphilis and a control line, wherein the detection line and the control line are arranged on the cellulose nitrate membrane in sequence; specific recombinant antigens of the syphilis are coated at the detection line of the specific IgG antibodies of the syphilis, and human IgG antibodies are coated at the control line; and horse radish peroxidase is marked on anti-human gamma-chain antibodies. The preparation method comprises the following steps: preparing the specific recombinant antigens of the syphilis, carrying out sample application on the cellulose nitrate membrane, and then preparing gamma-chain monoclonal antibodies of anti-human IgG specific segments; and marking the horse radish peroxidase on the anti-human IgG specific segments gamma-chain monoclonal antibodies, and then preparing the western blot kit. The invention can be used for detection of the specific IgG antibodies of the syphilis in specimens such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. In the invention, the amount of the needed specimens is very small, special instruments are not needed, the detection is simple, convenient and fast, the specificity is strong, the sensitivity is high, the accuracy and the reliability are achieved, and the cost is low.

Description

Syphilis-specific IgG antibody immunoblotting kit and preparation method thereof technical field The invention relates to a kit, in particular to a syphilis-specific IgG antibody detection immunoblotting kit and a preparation method thereof. Background technique Syphilis (Syphilis) is a sexually transmitted disease caused by Treponema pallidum (Treponemapallidum, TP). Treponema pallidum is mainly transmitted through sexual contact, blood transfusion, wound or placenta. Treponema pallidum enters the blood from the lymph nodes near the infected area and spreads throughout the body, involving almost all tissues and organs of the body. The World Health Organization (WHO) has optimistically predicted: "Syphilis is a sexually transmitted disease that can be successfully controlled through public health measures, thanks to highly sensitive detection methods and efficient treatment options." Regrettably, syphilis is still a worldwide public health problem without effective admin...

AI Technical Summary

Problems solved by technology

The commercially available Western-blot reagents are all imported reagents, which are expensive

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