Denitrified microbial agent as well as preparation method and application thereof

A microbial inoculant and denitrification technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting large-scale popularization and use, low investment cost, high investment and operation cost, and achieve short processing time. , reducing the total nitrogen index, the effect of broad application prospects

Inactive Publication Date: 2011-05-25
WUXI ZHONGJIA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the installation of denitrification devices in sewage treatment can reduce the total nitrogen index in water, the high investment and operation costs limit its large-scale promotion and use
The biological denitrification method only needs to add denitrification microbial agents in the traditional sewage treatment process, no equipment is required, and the investment cost is low; however, there are few reports on the research and application of microbial agents involved in biological denitrification in the sewage treatment process , there is no relevant report on the biological denitrification microbial agent specially used for sewage treatment in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of denitrification microbial bacterial agent:

[0035]Microbacterium oxydans MCCC 1A04 fermented liquid, Rhodococcus ruber ATCC 51882 fermented liquid and denitrifying bacterium Paracoccus denitrificans ATCC 17741 fermented liquid were respectively according to 30%, 20%, 50% proportioning (percentage is that the number of bacteria accounts for the percentage of the total number of bacteria) ) mixed with solid auxiliary materials, the mass ratio of solid auxiliary materials to fermentation broth is 1.5:1, stirred evenly, dried at 28°C, pulverized, sieved through 40 meshes, packaged and bagged. Wherein, the fermentation broth of Microbacterium oxydans MCCC 1A04, Rhodococcusruber ATCC 51882 and denitrifying bacteria Paracoccus denitrificans ATCC 17741 are all obtained through slant culture, primary seed culture and fermentation culture. The slant medium, primary seed culture and fermentation culture involved are all nutrient broth medium, the specific formula: ...

Embodiment 2

[0039] Preparation of denitrification microbial bacterial agent:

[0040] Microbacterium oxydans CGMCC 1788 fermented liquid, Rhodococcus ruber ATCC 15906 fermented liquid and denitrifying bacterium Paracoccus denitrificans ATCC 13543 fermented liquid were respectively according to 50%, 20%, 30% proportioning (percentage is the number of bacteria accounts for the percentage of total number of bacteria) ) mixed with solid auxiliary materials, the mass ratio of solid auxiliary materials to fermentation broth is 2:1, stirred evenly, dried at 40°C, pulverized, sieved through 40 meshes, packaged and bagged.

[0041] Among them, the fermentation liquid of Microbacterium oxydans CGMCC 1788, the fermentation liquid of Rhodococcus ruber ATCC 15906 and the fermentation liquid of denitrifying bacteria Paracoccus denitrificans ATCC 13543 were all obtained through slant culture, primary seed culture and fermentation culture. The slant medium, primary seed culture and fermentation culture i...

Embodiment 3

[0045] Preparation of denitrification microbial bacterial agent:

[0046] Microbacterium oxydans MCCC 1A04974 fermented liquid, Rhodococcus ruber ATCC 534 fermented liquid and denitrifying bacterium Paracoccus denitrificans ATCC 35512 fermented liquid were respectively according to 40%, 20%, 40% proportioning (percentage is that the number of bacteria accounts for the percentage of the total number of bacteria) ) mixed with solid auxiliary materials, the mass ratio of solid auxiliary materials to fermentation broth is 1.8:1, stirred evenly, dried at 35°C, pulverized, sieved through 40 meshes, packaged and bagged.

[0047] Among them, the fermentation liquid of Microbacterium oxydans MCCC 1A04974, the fermentation liquid of Rhodococcus ruber ATCC 534 and the fermentation liquid of denitrifying bacteria Paracoccus denitrificans ATCC 35512 were all obtained through slant culture, primary seed culture and fermentation culture. The slant medium, primary seed culture and fermentatio...

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Abstract

The invention discloses a denitrified microbial agent as well as a preparation method and application thereof. The denitrified microbial agent contains Microbacterium oxydans, Rhodococcus and Paracoccus denitrificans. The preparation method comprises the following steps of: uniformly mixing a Microbacterium oxydans fermentation liquor, a Rhodococcus fermentation liquor and a Paracoccus denitrificans fermentation liquor with solid auxiliary materials according to a ratio; drying at 28-40 DEG C; pulverizing; and sieving with 10-50 meshes. The denitrified microbial agent is in a dormant state atnormal temperature in vacuum, can be used for quickly and effectively reducing the total nitrogen index in water in a sewage treatment process, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a denitrification microbial bacterial agent and its preparation method and application. Background technique [0002] With the continuous development of modern industry, the widespread application of chemical fertilizers and the discharge of a large amount of domestic and industrial sewage, the nitrogen pollution in wastewater is becoming more and more serious. The nitrogen source in sewage is the nutrient of the water body, but the eutrophication of the water body can easily cause the water body to be black and smelly. Excessive algae and ammonia in the source water will increase the cost of water treatment. Combined nitrogen (such as nitrite and nitrate, etc.) has toxic side effects on humans and organisms, especially nitrite, which is a carcinogen, should be Strictly control emission standards. At present, the BOD, COD and TP of most sewage treat...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01
Inventor 朱宝泉胡海峰陈纪文张琴陈建昌赵晓鸣
Owner WUXI ZHONGJIA TECH
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