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Method for killing bacterial spores

A technology of spores and bacteria, applied in chemistry and other fields, can solve environmental pollution and other problems, and achieve the effects of convenient use, low corrosion and low cost

Inactive Publication Date: 2012-12-12
ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, when decontaminating the epidemic foci caused by bacterial spores, the conventional method is to adopt incineration and long-term immersion in high-concentration chemical preparations, but these methods pollute the environment very seriously

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  • Method for killing bacterial spores
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Embodiment Construction

[0015] Below in conjunction with example the present invention will be further described.

[0016] 1. Laboratory research results

[0017] With a final concentration of 0.10 mol\L L-alanine as the main component of the germination agent, act on the spore suspension for 20-60 minutes. Use sodium hydroxide alkali solution with a concentration of 0.02-0.50 mol\L as the resistance weakening agent, act on the above-mentioned spore suspension for 20-40min, and use H 2 o 2 , Fe 2+ , EDTA as the reaction system, determine the H 2 o 2 The final concentration of ferrous sulfate is 0.20mol / L, the concentration of ferrous sulfate is 0.02mol / L, the concentration of EDTA is 0.02mol / L, the pH value is 4.0-5.0, and the action time is 30-60min. The results are shown in Table 1.

[0018]

[0019] The spore germination agent, resistance weakening agent (alkalizing agent) and oxidizing agent of the present invention are formulated according to the method and used according to the sterili...

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Abstract

The invention relates to a method for killing bacterial spores. The method comprises the following steps: 1) dropping L-alanine serving as a spore germinator on a contaminated article; 2) then continuing dropping a strong alkaline solution serving as a resistance weakening agent; and 3) adjusting the pH value of the solution to 4.0-5.0 by taking H2O2, Fe2<+> and EDTA as a reaction system so as toprepare an oxidant; and dropping the prepared oxidant on the contaminated article. The method provided by the invention is used for disinfecting the contaminated environment and articles and killing the bacterial spores attached to the environment and articles, and has the advantages of good effect, low toxicity, little stimulus, low cost and the like.

Description

technical field [0001] The invention relates to a sterilization method, in particular to a method capable of effectively inactivating bacterial spores. technical background [0002] Bacterial spores are highly resistant to physical and chemical agents. Therefore, the conventional method for decontaminating the epidemic foci caused by bacterial spores is to incinerate and soak in high-concentration chemical preparations for a long time, but these methods pollute the environment very seriously. For example, an on-site anthrax test was carried out on Gruinard Island near the coast of Scotland in 1942. The island was seriously polluted. In 1986, it was purified by soaking in a mixture of high-concentration formaldehyde and seawater; High-volume bleaching powder is mixed with soil and soaked, and then burned with gasoline. [0003] In recent years, according to the characteristics of bioterrorism attacks, the attack sites are often in places with a high concentration of people,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/18A61L101/36A61L101/22A61L101/02A61L101/28
Inventor 熊鸿燕张林黄国荣林辉许斌
Owner ARMY MEDICAL UNIV
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