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Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage

A microbial fertilizer, food waste technology, applied in the direction of microorganisms, microorganism-based methods, organic fertilizers, etc.

Inactive Publication Date: 2011-05-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And the method of producing Trichoderma with food waste wastewater as culture medium has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Production of green trichoderma microbial fertilizer by liquid and solid combined fermentation of kitchen waste wastewater:

[0014] Seed preparation: Sterilize the kitchen waste wastewater at 115°C for 20 minutes, inoculate Trichoderma viride with an inoculation concentration of 107 spores / ml culture medium after cooling, and culture at 26°C for 36 hours in a shaker flask at 150 rpm to obtain the final product liquid seeds.

[0015] Liquid fermentation: Sterilize the kitchen waste wastewater at 115°C for 20 minutes, and the fresh wastewater may not be sterilized. Inoculate 5% liquid seeds, and culture at 26° C. in a fermenter for 36 hours with aerobic ventilation to obtain liquid Trichoderma viride product.

[0016] Solid-state fermentation: Using rice husk as a support, add the cultured liquid seeds or liquid Trichoderma products to the rice husk according to 2.5 times the weight, stir evenly, and culture statically at 26°C for 7 days to obtain solid Trichoderma viri...

Embodiment 2

[0019] Production of Trichoderma harzianum microbial fertilizer by liquid and solid combined fermentation of kitchen waste wastewater:

[0020] Seed preparation: Sterilize the kitchen waste wastewater at 121°C for 15 minutes, inoculate Trichoderma harzianum after cooling, at an inoculation concentration of 10 6 spores per milliliter of culture medium, cultured at 28°C, shake flasks at 150 rpm for 48 hours to obtain liquid seeds.

[0021] Liquid fermentation: Sterilize the kitchen waste wastewater at 121°C for 15 minutes, inoculate 10% liquid seeds, and culture in a fermenter at 28°C for 36 hours with aerobic aeration to obtain liquid Trichoderma harzianum products.

[0022] Solid-state fermentation: using beet pulp as a support, add the cultured liquid seeds or liquid Trichoderma products to the beet pulp according to 3 times the weight, stir evenly, and culture at 28°C for 6 days with static ventilation to obtain solid Trichoderma harzianum product.

[0023] Post-processing...

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PUM

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Abstract

The invention relates to a production method of a trichoderma spp. biocontrol agent, and belongs to technical fields of microbial fertilizers and the resource utilization of waste water. According to the method, grape peel dregs and grain stillages produced during grape wine production are used as raw materials for the production, and a method of solid state fermentation is adopted to produce the trichoderma spp. biocontrol agent. The production process comprises: (1) taking waste water of kitchen garbage as a medium to inoculate trichoderma spp. and preparing liquid seeds by liquid culture; (2) inoculating the liquid seeds to prepare a large number of liquid trichoderma spp. products with waste water of kitchen garbage as raw materials for the fermentation; (3) adding the cultured liquid seeds or the liquid trichoderma spp. products into biomass materials, and performing solid state fermentation to prepare solid trichoderma spp. products; (4) drying the fermented products, adding stabilizing agents and auxiliary agents, pulverizing, and packaging. The process for producing trichoderma spp. microbial fertilizers has low production cost and is environment-friendly; the products can significantly improve the quality of soil environment, increase crop yields, and control various plant diseases caused by fungi. A processing method for effectively processing plenty of high concentration waste water produced during the production of kitchen garbage is simultaneously realized and environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizer and waste water resource utilization. technical background [0002] With the continuous development of my country's economy and the continuous improvement of people's living standards, the domestic catering industry has entered a stage of rapid development. The 1.3 billion people consume a huge amount of food, and the resulting food waste is also increasing. There is more than 100,000 tons of kitchen waste every day. If it is not handled properly, it will easily cause serious pollution to the environment; The processed food waste is directly fed to livestock and poultry. Since the food waste is easy to rot, there is a high food safety risk in this utilization method. At present, Beijing, Shanghai, Shijiazhuang, Chongqing, Yinchuan, Ningbo, Jingdezhen, Shenzhen, Suzhou and other large and medium-sized cities have issued "Management Measures" or drafts related to the collection, transp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N1/14C12R1/885
Inventor 白志辉宿燕明荆梦徐圣君彭霞薇张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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