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Method for preparing purified alkaloid part in Chinese angelica-szechuan lovage rhizome supercritical CO2 extract

A supercritical, extractive technology, applied in the field of alkaloid parts, can solve problems such as few research literature reports

Inactive Publication Date: 2011-05-04
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the reports in the literature are limited to the research on the alkaloids and ligustrazine in the single medicine of Chuanxiong, and there are few reports on the alkaloids and ligustrazine in the combination of "Angelica-Chuanxiong" medicine

Method used

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  • Method for preparing purified alkaloid part in Chinese angelica-szechuan lovage rhizome supercritical CO2 extract
  • Method for preparing purified alkaloid part in Chinese angelica-szechuan lovage rhizome supercritical CO2 extract
  • Method for preparing purified alkaloid part in Chinese angelica-szechuan lovage rhizome supercritical CO2 extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take "Angelica-Chuanxiong" supercritical CO 2 The extract of the total alkaloid part is extracted with petroleum ether (boiling range 30-60°C) to remove the small polar part, and the supercritical CO 2 For the alkaloid part in the extract, adjust the pH value of the upper column liquid to 8, and measure the total alkaloid content in it to be 40%, and the mass concentration of Ligustrazine to be 200mg / mL; put it on the column macroporous adsorption resin AB-8, and the above column flow rate 2BV / h sample loading, 35% ethanol volume concentration, elution flow rate 1BV / h elution, collect drug-containing eluent, recover ethanol at a lower temperature, and obtain purified alkaloid fraction, acid-base dye colorimetric method Its total alkaloid content is measured to be: 58%, wherein, ligustrazine is accounted for 37% as determined by high performance liquid chromatography.

Embodiment 2

[0036] Freeze-dry the purified alkaloid parts obtained in Example 1 to obtain "Angelica-Chuanxiong" supercritical CO 2 Lyophilizate of the purified alkaloid fraction from the extract.

Embodiment 3

[0038] Take "Angelica-Chuanxiong" supercritical CO 2 The extract of the total alkaloid part is extracted with petroleum ether (boiling range 60-90°C) to remove the small polar part, and the remaining "Angelica-Chuanxiong" supercritical CO 2 For the alkaloid part in the extract, adjust the pH value of the upper column liquid to 10, and measure the total alkaloid content in it to be 52%, and the mass concentration of Ligustrazine to be 320mg / mL; put it on the column macroporous adsorption resin HPD100, and the above column flow rate is 4BV / h loading sample, ethanol volume concentration 85%, elution flow rate 2BV / h elution, collect drug-containing eluate, recover organic solvent at lower temperature, obtain purified alkaloid part, acid-base dye colorimetric method Its total alkaloid content is: 65%, wherein, ligustrazine is accounted for 36% as determined by high performance liquid chromatography.

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Abstract

The invention discloses a method for preparing a purified alkaloid part in 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract, which comprises the following steps of: a) extracting total alkaloid parts serving as raw materials in the 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract by using an organic solvent so as to obtain an alkaloid part in the 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract; and b) eluting the alkaloid part serving as column loading liquid in the 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract in the step a) through macroporous absorption resin by using an eluant, collecting the medicine-containing eluant and recovering the eluant so as to obtain the purified alkaloid part in 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract. The provided purified alkaloid part in the 'Chinese angelica-szechuan lovage rhizome' supercritical CO2 extract has the characteristics of high content of active ingredient, and the like.

Description

technical field [0001] The invention relates to the field of traditional Chinese herbal medicine, in particular to the supercritical CO 2 Process for the preparation of purified alkaloid fractions from extracts, and alkaloid fractions produced by the process. The invention also relates to the freeze-dried product obtained by freeze-drying the alkaloid fraction. The present invention also relates to the application of the alkaloid fraction or the freeze-dried product in the preparation of anti-myocardial ischemia, anti-thrombotic, blood-lipid-lowering and atherosclerosis-improving drugs. Background technique [0002] Angelica sinensis is the dried root of Angelica sinerisis (Oliv.) Diels of Umbelliferae. Angelica belongs to the Liver, Heart, and Spleen Meridians, and its medicinal properties are sweet, pungent, and warm. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and defec...

Claims

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Application Information

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IPC IPC(8): A61K36/236A61P3/06A61P7/02A61P9/10
Inventor 杨义芳冯敬骞
Owner SHANGHAI INST OF PHARMA IND
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