Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon

A technology of Penaeus monodon and Bdellovibrio, which is applied in the fields of application, bacteria, and fish farming, can solve the problems of adverse effects on the immunity of shrimp organisms, drug resistance, drug residues, and slow effect, so as to improve the disease resistance of shrimp ability, prevention of vibriosis in prawns, and improvement of survival rate

Inactive Publication Date: 2011-05-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese herbal medicine has the advantages of no drug residue and high economic benefit on the growth performance of Penaeus monodon, but has the disadvantage of slow effect
Antibiotics have played a positive role in inhibiting the reproduction of pathogenic microorganisms and reducing the toxicity of their metabolic toxins to shrimp. However, the long-term use of antib

Method used

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  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon
  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon
  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 Bdellovibrio bacteroid bacterium liquid

[0024] Described hiruplasma obtains by following method: in containing Vibrio parahaemolyticus (inoculation concentration is 10 7 cfu / mL bacteria 1mL) (Vibrio parahaemolyticus, purchased from American Type Culture Collection, ATCC 17802) 1L DNB (dilute nutrient broth) liquid medium (nutrient broth 0.8g, casein acid hydrolyzate 0.5 g, yeast extract 0.1g, dissolved in 1000mL distilled water, pH value is 7.6) into 1mL concentration of 10 3 pfu / mL Bdellovibrio, cultured at 28°C and 250rpm for 48h, the culture solution was centrifuged at 6000rpm at 4°C for 15min, the supernatant was removed, and the precipitate was suspended with 100mL DNB to obtain the Bdellovibrio; concentration up to 10 9 pfu / mL.

Embodiment 2

[0025] Example 2 The application experiment of Bdellovibrio bdelloplast bacterium liquid in common cultivation of Penaeus monodon

[0026] The specific implementation site of the present invention is a certain breeding farm in South China, and the implementation time is 3 months.

[0027] (1) Conditions of the breeding pond, the bottom area of ​​the breeding pond is 10 mu, the depth of the pond is 2m, the bottom of the pond is sandy, and the bottom of the pond is flat. system;

[0028] (2) Preparation before stocking. The water quality of the water source in the sea area of ​​the test site meets the requirements of the National Standard Fishery Water Quality Standard of the People's Republic of China (GB11607-89). Aquaculture Water Quality" (NY5052-2001) requirements. 10 days before putting the seedlings, filter the water with an 80-mesh sleeve net, and apply nitrogen, phosphorus, and potassium compound fertilizers to cultivate the bait organisms at the same time, so that t...

Embodiment 3

[0111] Example 3 The application experiment of Bdellovibrio bdelloplasts in culture of Penaeus monodon in elevated ponds

[0112] (1) The structure of the shrimp pond is 88m×83m×2.2m, the four corners are rounded, and the area of ​​each shrimp pond is 10 mu, which is arranged in a horizontal line in the east-west direction. The distance between the shrimp ponds is 5m, and the altitude is 10m. Cement concrete slope protection is used around the shrimp ponds, and a plastic film is laid on the bottom of the ponds, and then 35cm of sand is covered on the film.

[0113] Wells are dug at the lowest low tide line in the sea area, and pipes are laid to the pump. The distance between the rooms is 72m and 100cm concrete pipes are used. The pump room uses 3 pumps of model 8BA18 with a power of 22kW / unit to pump water to the shrimp pond. The distance is 110m. 60cm×70cm open channel, and then divert water into each shrimp pond through 40cm×60cm open channel. A water wheel aerator is used,...

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Abstract

The invention in particular relates to an application of a bdellovibrio sp. leech plasmid in cultivating marine products such as penaeus monodon, and the like. The invention is realized by adding a bdellovibrio sp. leech plasmid to the water body and the feeds. The invention is realized specifically based on cultivation pond conditions, preparation before stocking, shrimp postlarvae selecting andstocking, feeding management and water environment management in the cultivation period. The invention is characterized by adding a bdellovibrio sp. leech plasmid bacterium solution to the water bodyand ensuring the concentration of the bdellovibrio sp. leech plasmid in the water body to be 101-107pfu/mL, simultaneously soaking the feeds in the bdellovibrio sp. leech plasmid bacterium solution with the concentration of 101-107pfu/mL for 30 minutes and changing water once every 30 days in the cultivation period. The invention provides a method for cultivating organisms. The method has the advantages of environment friendliness, safety, no pollution, obvious effect and capabilities of improving the water quality, promoting growth of penaeus monodon, improving the immunity and improving thesurvival rate, thus having wonderful development prospect. The invention has very important significance in improving the economic benefits and eating safety and guaranteeing human health.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a bacterium, in particular to the application of the bdellovibrio bruplasma liquid in the cultivation of marine products such as Penaeus monodon. Background technique [0002] Penaeus monodon (Penaeus monodon) is commonly known as ghost shrimp, grass shrimp, flower shrimp, bamboo prawn, monodon prawn, bovine prawn, and the Food and Agriculture Organization of the United Nations is commonly known as tiger prawn. It is the largest individual among prawns. The mature individual generally has a body length of 22.5cm to 32cm and a weight of 137g to 211g. It is one of the common varieties of shrimp farming in the world today. [0003] The current non-polluting breeding method causes organic waste such as shrimp excreta, residual feed, and plankton residues to accumulate in the pond. In the middle and late stages of farming, bottom pollution will inevitably occur, water qua...

Claims

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Application Information

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IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏王沛贤
Owner SOUTH CHINA UNIV OF TECH
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