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Kit for synchronously separating and identifying salmonella and shigella as well as preparation and application

A simultaneous separation and Salmonella technology, applied in biochemical equipment and methods, microbial measurement/inspection, resistance to vector-borne diseases, etc., can solve the problems of complicated identification steps, high missed detection rate, high cost, etc., to improve timeliness and accuracy, ease of mastery and acceptance, and the effect of reducing random errors

Inactive Publication Date: 2011-04-27
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology

It mainly solves the technical problems of high missed detection rate, complicated identification steps and high cost, and unintuitive screening results existing in the existing methods for the isolation of Salmonella and Shigella

Method used

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  • Kit for synchronously separating and identifying salmonella and shigella as well as preparation and application

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Embodiment Construction

[0032] First prepare the kit according to the content of the technical plan, and the user chooses to use different enrichment solutions or selective plates for combined separation according to different test materials, and finally uses simple biochemical and specific enzyme-substrate reaction tests to differentially diagnose Salmonella and Shigella (Table 2).

[0033] 1. Explanation, collection requirements and preprocessing methods of different inspection materials:

[0034] 1) Patient's blood: Take 3-7ml of patient's venous blood by aseptic operation, immediately inoculate it in a commercial blood culture bottle for enrichment culture and at the same time inoculate xylose lysine deoxycholate agar plate (XLD) and Salmonella Chromogenic agar plate (CAS) for direct isolation, cultured at 36°C for 24h-48h to observe the results of colony growth. If it is clotted blood, the blood clot is crushed and inoculated. If the commercial blood culture system shows a positive result, pic...

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Abstract

The invention relates to a method for synchronously separating and identifying salmonella and shigella in enteric pathogenic bacteria, which mainly solves the technical problems of high detection-missing rate, complicated identification step, high cost and unintuitive screening result in the existing method for separating the salmonella and the shigella. The kit comprises: 1) a TSB (Trypticase Soy Broth) serving as an universal-type non-selective proliferous liquid; 2) a 10-folds concentrated TSB serving as a 10-folds concentrated universal-type non-selective proliferous liquid; 3) a selenite brilliant green-sulfanilamide proliferous liquid serving as a salmonella proliferous liquid; 4) a shigella proliferous liquid; 5) a xylose lysine deoxycholate agar plate; 6) a salmonella chromogenic agar plate; 7) a salmonella-shigella comprehensive biochemical identification tube comprising a first test tube and a second test tube; 8) a hydrogen sulphide filter paper strip; and 9) an indole filter paper strip. In the invention, typical colonial morphology corresponding to the growth of a selective isolation medium, simple biochemistry and reaction combination test of a specific enzyme and a substrate are adopted to identify two kinds of enteric pathogenic bacteria.

Description

technical field [0001] The present invention relates to a method for synchronous isolation and identification of Salmonella and Shigella among enteric pathogenic bacteria, in particular to the identification of two species using the typical colony morphology corresponding to the growth of selective isolation medium, simple biochemical and specific enzyme-substrate reaction combination tests Kits and methods of use for enteropathogenic bacteria. Background technique [0002] The routine isolation methods for Salmonella and Shigella currently used in actual work require cumbersome inter-batch quality control requirements, low sensitivity and specificity, and low sensitivity and specificity for traditional isolation media. Expensive. Chinese patent application 200810047994.7 discloses "a method for rapid detection of Salmonella and Shigella at the same time", which belongs to the rapid detection technology of food-borne pathogens, specifically a method for simultaneous detecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCY02A50/30
Inventor 金汇明许学斌
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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