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Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide

A technology of camellia polysaccharides and tea polysaccharides, which is applied in the field of medicinal applications of the camellia polysaccharides, can solve the problems that the research on the development and utilization of tea tree flowers has not yet been fully carried out, and achieve the effect of simple preparation process

Inactive Publication Date: 2011-04-27
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, people's current research on the utilization of tea mainly involves the role of tea in food, medicine and human health care, while the research on the development and utilization of tea tree flowers has not yet been fully carried out. A large number of camellias can only fall off and return to nature and soil. mix

Method used

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  • Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 10Kg of dried camellia was fed, crushed through a 16-mesh sieve, added 80L of water, stirred, extracted at room temperature for 30 minutes, and centrifuged at 4500 rpm to separate the solid from the liquid and collect the extract. Add 60L of water to the solid precipitated camellia, stir and extract at room temperature for 30 minutes, centrifuge at 4500 rpm to separate the solid and liquid, combine the extract with the first extract, and discard the solid precipitated camellia.

[0027] The combined extracts were concentrated under reduced pressure at 75 degrees Celsius to a density greater than 1.2Kg / L, and after cooling, 4 times the volume of ethanol was added for precipitation for 12 hours. The supernatant was recovered with ethanol, and the precipitate was dried under reduced pressure and vacuum at 75°C. Camellia crude polysaccharide 1.68Kg was obtained.

Embodiment 2

[0029] 10Kg of dried camellia was fed, crushed through a 16-mesh sieve, added with 80L of water, stirred, extracted with a microwave at 600w for 10 minutes, filtered with a plate and frame, and the extract was collected. Add 60L of water to the solid camellia, extract with 600w microwave for 10 minutes, filter the plate and frame to separate the solid and liquid, combine the extract with the first extract, and discard the solid precipitated camellia.

[0030] The combined extracts were concentrated under reduced pressure at 75 degrees Celsius to a density greater than 1.1Kg / L, and after cooling, 4 times the volume of ethanol was added to precipitate for 12 hours. The supernatant was recovered with ethanol and freeze-dried in vacuum. Camellia crude polysaccharide 211g was obtained.

Embodiment 3

[0032] The crude polysaccharide from camellia obtained in Example 1 was dissolved in water, and the ion exchange resin RJA (purchased from Shanghai Mosu Scientific Equipment Co., Ltd.) column was applied at a flow rate of 3 times the column volume per hour, and the tea polysaccharide was eluted after loading the sample, with a yield of 82 %, the decolorization effect is good.

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Abstract

The invention relates to a method for extracting and preparing camellia polysaccharide from a camellia and an application of the prepared camellia polysaccharide. The method comprises the following steps: extracting with preferable water or enzyme-containing water, carrying out alcohol precipitation on the extracting solution, separating, drying the separated precipitate to obtain camellia coarse polysaccharide, decoloring and deproteinizing the camellia coarse polysaccharide by resin or a chemical method, and purifying with gel or an ultrafiltration membrane to obtain the camellia polysaccharide. Simultaneously, the animal experiment proves that the camellia polysaccharide has the effect of preventing and treating diabetes.

Description

technical field [0001] The invention belongs to the technical field of food, health food or medicine preparation, and in particular relates to a method for extracting and preparing camellia polysaccharide from camellia, and relates to the medical application of the camellia polysaccharide. Background technique [0002] Camellia is one of the reproductive organs of the tea tree. The tea tree begins to differentiate flower buds in May every year, blooms from September to October, and the flowering period lasts 100 to 110 days. The flowering period of tea trees in the southern tea areas of my country is relatively long, which can last until February to March of the following year, and in some areas even the camellias can be seen blooming throughout the year. The national tea area can produce more than 3 million tons of camellia every year, and the resources are quite rich. [0003] Studies have shown that Camellia is rich in polysaccharides, proteins, amino acids, vitamins an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10C12S3/02
Inventor 张华王元凤茅仁刚魏新林袁萍
Owner SHANGHAI NORMAL UNIVERSITY
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