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Extract of effective parts of total saponins in pterocephalus hookeri as well as preparation method and application thereof

A technology of effective parts and total saponins, which is applied in the field of extracts and preparations of effective parts of total saponins of pterygium, can solve problems such as the extraction and purification of total saponins of pterygium that have not yet been seen, and achieve inhibition of tumor cell proliferation and economical purification process Effect

Inactive Publication Date: 2012-08-29
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, there are no reports on the extraction and purification of the effective fraction of total pterygium saponins and its pharmacodynamics in antitumor.

Method used

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  • Extract of effective parts of total saponins in pterocephalus hookeri as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Weigh 60g of crushed pterygium root, immerse in 80% ethanol for 12h, and then heat extract 3 times, each time the amount of solvent is 5-12 times, each extraction time is 60min, combine 3 extracts, concentrate to 60ml, use 25 Dilute to 120ml with % ethanol, pass through 50ml of AB-8 type macroporous resin at a flow rate of 1.5BV / h, eluted with 200ml of water to remove impurities, then eluted with 200ml of 60% ethanol, collected the eluate, concentrated to a thick extract , and vacuum-dried for 4 hours to obtain the active part extract of Pterocephalus total saponins of the present invention, weighing 2.98g, with a total saponin content of 80%.

Embodiment 2

[0029] Weigh 60g of crushed pterygium root, immerse in 70% ethanol for 12h and heat extract 3 times, each time the amount of solvent is 5-12 times, each extraction time is 90min, combine 3 extracts, concentrate to 60ml, use 25 % ethanol is diluted to 120ml, passes through 50mlX-5 type macroporous resin with the flow velocity of 4BV / h, removes impurity with 250ml water elution successively, then with 250ml 70% ethanol elution, collects eluate, concentrates to thick extract, Vacuum dried for 4 hours to obtain the effective part extract of total saponins of Pterocephala of the present invention, weighing 2.65 g, with a total saponin content of 65%.

Embodiment 3

[0031] Weigh 60g of crushed pterygium root, immerse in 80% ethanol for 12h, then heat extract 3 times, each time the amount of solvent is 5-12 times, each extraction time is 120min, combine 3 extracts, concentrate to 60ml, use 25 Dilute to 120ml with % ethanol, pass through 50ml of HPD-100 type macroporous resin at a flow rate of 0.75BV / h, wash with 300ml of water in turn to remove impurities, then wash with 250ml of 80% ethanol, collect the eluate, concentrate to a thick extract , and vacuum-dried for 4 hours to obtain the active part extract of Pterocephalus total saponins of the present invention, with a weight of 2.8 g and a total saponin content of 60%.

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Abstract

The invention belongs to the field of pharmacy of traditional Chinese medicines, relating to an extract of effective parts of total saponins in pterocephalus hookeri as well as a preparation method and application thereof. By taking the content of the total saponins in the pterocephalus hookeri as an index, the extract of the total saponins in the pterocephalus hookeri provided by the invention has the characteristics of high content of the total saponins, controllable and stable quality and obvious drug effect. The main component of the extract is oleanane type pentacyclic triterpene saponins, and the content of the total saponins is 50%-90%. The preparation method provided by the invention is economical and simple in extraction technique and is applicable to industrial production. The obtained extract has obvious function of restraining the tumor cell proliferation, and can be applied in preparation of anti-tumor drugs.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to an extract of effective parts of total saponins of pterygium, a preparation method and application thereof. Background technique [0002] Pteroptera is a commonly used medicinal material in Tibetan medicine, and is widely used in Tibetan medicine preparations, such as Shishierwei Yishou Powder, Jiebai Pills, Erwuwei Ganzi Pills, Erwuwei Donkey Blood Pills, Twenty-six Empas Weiqingpeng Powder, Pomegranate Pu'an Powder, Qingfei Zhike Pill, etc. Tibetan medicine believes that pterygium is cold in nature, bitter in taste, and slightly poisonous. It has the functions of clearing away heat and detoxification, dispelling rheumatism, and relieving pain. It can be used to treat diseases such as blast poison, arthralgia syndrome, dysentery, and arthritis. At present, there are few reports on the research and application of Pterocephalus, and related literatures include: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61K31/56A61K36/185A61P35/00A61K125/00
Inventor 朱国福崔文霞雷旭东尹胜利
Owner SHANGHAI UNIV OF T C M
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