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Quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper

A cotton verticillium wilt, linerless technology, applied in the field of phytopathology, can solve the problems of test failure, the susceptible control does not meet the identification standard, etc., and achieves the effects of uniform root injury, short identification period, and sufficient disease incidence.

Active Publication Date: 2011-04-20
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the identification of Verticillium wilt resistance in national cotton variety regional tests and cotton variety regional tests in various provinces mostly uses artificial disease nurseries or natural disease fields for identification of adult plants. Since cotton verticillium wilt begins to occur in early June, it will last until August. At the end of the month and early September, the peak incidence of the whole year is reached, so the identification period is as long as about 150 days
Moreover, the occurrence of Verticillium wilt in cotton is affected by various environmental conditions such as soil bacteria content, temperature, humidity, and light. When the soil bacteria content is low or in high temperature and drought years, the occurrence of Verticillium wilt is light, and the susceptible control does not meet the identification standards. (Illness index 40.0~66.7), resulting in test failure

Method used

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  • Quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper
  • Quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper
  • Quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper

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Experimental program
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Effect test

Embodiment Construction

[0025] 1. Make a nutrition bowl

[0026] Mix the purchased vermiculite and sun-dried river sand evenly at a ratio of 6:4, make a 6cm×10cm paper tube with newspaper, and use a stapler to fasten both ends of the paper tube tightly to prevent the paper bowl from loosening. Put the paper tubes into a 75cm×35cm×15cm (length×width×height) plastic tray, with 5 bowls in each row, 6 rows in total, 3 rows at each end of the tray, with a distance of 15cm in the middle. Put the mixture of vermiculite and sand into the paper tube, 2cm away from the upper edge, to make the vermiculite sand nutrition bowl.

[0027] 2. Watering and preparing for sowing

[0028] Irrigate water from the gap in the middle of the plate so that the vermiculite sand in the nutrition bowl can fully absorb water. It is advisable that the top vermiculite sand in the paper bowl is moist and there is no accumulated water in the plate.

[0029] 3. Seed treatment and sowing

[0030] The seeds to be tested were develore...

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Abstract

The invention relates to the plant pathology field, and specially relates to a quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper. The method in the invention includes the following steps: 1) making a nutrient bowl by mixing vermiculite and sandy soil and putting them into a paper bowl; 2) sowing and culturing cotton sprouts; 3) inoculating pathogen, which requires inoculating bacterium spore suspension of cotton verticillium wilt when one true leaf of a cotton sprout grows to be flat, wherein the inoculation concentration is in the range of from 1*10<6> to 5*10<6> spores per ml, and every cotton sprout is inoculated with bacteria liquid about from 2 to 3 ml; 4) evaluating the resistance to cotton verticillium wilt. The invention is characterized by being fast, accurate, economical, environmental beneficial and so on.

Description

technical field [0001] The invention relates to the field of phytopathology, in particular to a method for quantitatively dipping bacterial solution in a vermiculite sandy soil bottomless paper bowl for identifying cotton Verticillium wilt resistance. Background technique [0002] Cotton verticillium wilt is one of the important diseases of cotton worldwide, and breeding disease-resistant varieties is one of the most economical and effective measures to control cotton verticillium wilt. Verticillium wilt resistance identification of resource materials and cotton varieties is the basic work of disease resistance breeding. At present, the identification of verticillium wilt resistance in national cotton variety regional tests and cotton variety regional tests in various provinces mostly uses artificial disease nurseries or natural disease fields for identification of adult plants. Since cotton verticillium wilt begins to occur in early June, until August At the end of the mon...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
Inventor 朱荷琴冯自力赵丽红师勇强李志芳
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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