Plant root development related protein ZmNR1 and coding gene thereof
A technology that encodes genes and proteins, applied in the field of genetic engineering, can solve problems such as the effect of fertilization on food crops to increase production, the reduction of nutrient utilization efficiency of chemical fertilizers, ecological problems, and environmental pollution
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Embodiment 1
[0063] Example 1. Discovery and expression analysis of maize MADS-box transcription factor ZmNR1 gene
[0064] 1. Discovery of the ZmNR1 gene
[0065] Using the homologous cloning strategy, according to the conserved domain M region of the MADS-box transcription factor ANR1 (Zhang and Forde, 1998, Science, 279:407-409) involved in the regulation of lateral root elongation in the model plant Arabidopsis The 80 amino acid sequences of the gene were homologously aligned in the NCBI database, and the maize mRNA sequence EU976250 with a similarity of 96% was obtained. Maize mRNA sequence EU976250 is the mRNA sequence of maize MADS-box transcription factor ZmNR1. The mRNA sequence of ZmNR1 consists of 681 bases, and its open reading frame (ORF) is 230-499 bases from the 5' end, encoding a MADS-box transcription factor protein containing 89 amino acids, and its molecular weight is 65KD, etc. Electric point 8.15.
[0066] figure 1 Figure A shows the phylogenetic analysis of ZmNR1....
Embodiment 2
[0102] Embodiment 2, cloning of maize ZmNR1 gene and its application in promoting root system development
[0103] 1. Cloning of the open reading frame sequence of the maize ZmNR1 gene
[0104] 1. Extract the extraction of total RNA from the root system of corn inbred line B73 seedling stage
[0105] Corn inbred line B73 (maize inbred line B73 was bred by Iowa State University, USA, and the B73 seeds used in this experiment were donated by Professor Schnable of Iowa State University, Schnable et al., The B73 Maize Genome: Complexity, Diversity, and Dynamics , Science 2009: 1112-1114, publicly available from China Agricultural University). The total RNA of the water culture root system at the seedling stage was extracted with the TRIzol Total RNA Extraction Kit (Takara Company), and the operation was performed according to the instructions of the kit. Specific steps are as follows:
[0106] Take 100mg of fresh corn root tissue and grind it in liquid nitrogen; add 1ml of the ...
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