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Novel preparation method of yolk diluent

A preparation method and technology of diluent, applied in the preparation field of egg yolk diluent, can solve the problems of time-consuming, labor-intensive, high cost, etc., and achieve the effects of improving the quality of frozen semen products, ensuring the viability rate, and saving manpower and material resources.

Active Publication Date: 2012-11-07
内蒙古赛科星繁育生物技术(集团)股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because egg yolk contains a large amount of organic components, it is not only time-consuming and labor-intensive, but also expensive to filter with a 0.22 μm sterile filter, so this technology was developed

Method used

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  • Novel preparation method of yolk diluent
  • Novel preparation method of yolk diluent
  • Novel preparation method of yolk diluent

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Experimental program
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Effect test

Embodiment

[0021] Embodiment: a kind of preparation method for the yolk dilution liquid that bovine frozen semen is produced, it comprises the following steps:

[0022] 1. Preparation of yolk: select fresh eggs, disinfect the eggshells with 75% (v / v) alcohol, remove the eggshells and egg whites under aseptic conditions, suck the egg whites on the surface of the yolk membrane with sterilized filter paper, and use Egg yolk was extracted with a sterile syringe;

[0023] 2. Preparation of 4.6 times Tris-A and 6 times Tris-B mother liquor:

[0024] Table 1 100ml 4.6×Tris-A mother solution formula

[0025] Drug Name

Weight (g)

Tris base

16.25

citric acid

7.92

D-Fructose

5.82

[0026] Table 2 100ml 6×Tris-B mother solution formula

[0027] Drug Name

Weight (g)

Tris base

21.45

citric acid

11.99

D-Fructose

8.83

[0028] Prepare 4.6 times Tris-A mother liquor and 6 times Tris...

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Abstract

The invention discloses a novel preparation method of yolk diluent, belonging to the field of animal reproductive biology. The novel preparation method comprises the following steps of: preparing yolk: preparing 4.6 times of Tris-A mother solution and 6 times of Tris-B mother solution and carrying out the aseptic filtration; diluting into 0.5 time of Tris-A mother solution and Tris-B mother solution with sterile water; respectively mixing with the yolk, stirring, standing, centrifuging and obtaining supernatant; respectively adjusting the concentration of the supernatant with 4.6 times of Tris-A mother solution and 6 times of Tris-B mother solution to obtain 1 time of Tris-A mother solution and Tris-B mother solution; and respectively adding antibiotic and sterile glycerin, adjusting the pH value and storing the mixture at 4DEG C for later use. The method omits the filtering step and is simple; fewer granules exist in the yolk diluent; and the method ensures the motility rate of cryopreservation sperms, improves the quality of froze sperm products and reduces the production cost.

Description

Technical field: [0001] The invention belongs to the technical field of animal reproduction, is applicable to the preparation of diluent in the production of bovine semen, and mainly provides a preparation method of yolk diluent. Background technique: [0002] Diluent is the main and necessary reagent in the production of bovine frozen semen, and yolk diluent is widely used in the production of bovine sex-controlled frozen semen because it has a better protective effect on sperm. There are many particles and impurities in the egg yolk dilution that affect the quality of the dilution and the detection of the product. At present, these impurity particles are mainly removed by centrifugation and filtration. Because egg yolk contains a large amount of organic components, filtering with a 0.22 μm sterile filter is not only time-consuming and labor-intensive, but also expensive, so this technology was developed. Invention content: [0003] The invention provides a method for pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 周文忠王丽霞胡树香李喜和
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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