In situ hybridization detection kit of KiSS1 gene and detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of heterogeneity, tumor cell drug resistance, failure of the anti-cancer battle, etc., and achieve the effects of high sensitivity, strong specificity and convenient operation.

Inactive Publication Date: 2011-03-30
SUZHOU FUYING GENE TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug resistance of tumor cells; 3. Incomplete design of anticancer drugs, etc.
The inventor found in the research that the other two important reasons for the non-declination of cancer mortality are: 1. Can not achieve real early diagnosis; 2. The pathological mechanism of metastasis is not clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In situ hybridization detection kit of KiSS1 gene and detection method and application thereof
  • In situ hybridization detection kit of KiSS1 gene and detection method and application thereof
  • In situ hybridization detection kit of KiSS1 gene and detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] An in situ hybridization detection kit for KiSS1 gene, comprising a hybridization probe, a marker, and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO. 1. The hybridization probe is labeled with digoxin. The composition of other liquids and specimens in the kit is as follows:

[0046] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0049] Justice Hybridization Solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Sealing solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0052] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0053] Color developer A 175μl / tube 1 tube / box yellow liquid

[0054] Color developer ...

Embodiment 2

[0096] A Kind of KiSS1 Gene In Situ Hybridization Detection Method and Its Kit Application

[0097] 1. Specimen processing

[0098] 1. Use a 10ml centrifuge tube to pack 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulant blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), centrifuge at 2000 r / min 10min;

[0099] 2. Pipette the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× Buffer I to this tube, mix well, and centrifuge at 1500g / min for 10 minutes;

[0100] 3. Discard the supernatant. Add about twice the amount of 1× Buffer I to the pellet, mix well, and centrifuge at 1500g / min for 10 minutes;

[0101] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with a paper towel. The precipitate is then made into a suspension, dropped on the glass slide to push the slide, and dried naturally. (Hospita...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an in situ hybridization detection kit of a KiSS1 gene, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is as shown by SEQ ID NO.1. The invention also provides an in situ hybridization detection method of the KiSS1 gene. Besides, the invention also provides application of the kit in preparing a medicament for detecting early metastasis of cancer and recurrent diseases. The invention has the following advantages that: the kit has the characteristics of high flexibility and strong specificity. The detection method of the invention is convenient and simple to operate, and can be commonly used and popularized in hospitals above district level.

Description

【Technical Field】 [0001] The invention relates to a kit, in particular to a kit for in situ hybridization detection of KiSS1 gene and its detection method and application 【Background technique】 [0002] In 2005, the U.S. Institutes of Health, the Cancer Institute, the Centers for Disease Control and Prevention made an annual report, "Thinking that human beings have failed in the fight against cancer." That is to say, the cancer mortality rate has not decreased. Several factors for the failure of the cancer battle are: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete anti-cancer drug design ideas. At the same time, the report also proposed that existing measures for the diagnosis and treatment of cancer should be reviewed. The inventors discovered in the research that two other important reasons leading to the non-reduction of cancer mortality are: 1. Real early diagnosis cannot be achieved; 2. The pathological mechanism of metastasis is unclear. Accordi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner SUZHOU FUYING GENE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap