In-situ hybridization detection kit and detection method for PTEN gene, and application of kit

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of nutrient consumption and the like, and achieve the effects of convenient operation, radical cure of malignant diseases of cancer and high sensitivity

Inactive Publication Date: 2011-03-23
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that most of the cells in the human body can proliferate, but this hyperplasia is limite...

Method used

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  • In-situ hybridization detection kit and detection method for PTEN gene, and application of kit
  • In-situ hybridization detection kit and detection method for PTEN gene, and application of kit
  • In-situ hybridization detection kit and detection method for PTEN gene, and application of kit

Examples

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Embodiment 1

[0046] An in situ hybridization detection kit for PTEN gene, comprising a hybridization probe, a marker and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0047] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0049] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0050]Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0052] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0053] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0054] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0055] Chrom...

Embodiment 2

[0097] A kind of PTEN gene in situ hybridization detection method and its kit application

[0098] 1. Specimen processing

[0099] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0100] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0101] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0102] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with conditi...

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Abstract

The invention relates to an in-situ hybridization detection kit for a PTEN gene. The kit comprises a hybridization probe and a marker, wherein the hybridization probe has a sequence shown as SEQ ID NO.1. The invention also provides an in-situ hybridization detection method for the PTEN gene. In addition, the invention provides application of the kit to the preparation of medicaments for detecting cancers. The invention has the advantages that: the kit has the characteristics of high sensitivity and specificity; and the detection method is convenient and easy to operate and can be universally used and popularized in hospitals of district-level or above.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to a PTEN gene in situ hybridization detection kit and its detection method and application 【Background technique】 [0002] Cancer is also called malignant tumor. Tumor refers to a local mass formed by the abnormal proliferation of cells in local tissues under the action of various tumorigenic factors. Benign tumors are easy to remove, generally do not metastasize or recur, and only squeeze and block organs and tissues. However, malignant tumors can also destroy the structure and function of tissues and organs, cause necrosis, hemorrhage and infection, and the patient may eventually die due to organ failure. [0003] The basic unit of a cancer lesion is the cancer cell. After human cells age and die, new cells will replace them to maintain body functions. It can be seen that most cells in the human body can proliferate, but this hyperplasia is limited, while the proliferation of cancer cells i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner NATUREGEN BIOTECH SHANGHAI
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