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Method for identifying hericium erinaceus strain king by utilizing molecule marker technology

A technology of molecular markers and technical identification, applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve the difficulties in standardizing production and product quality standards, the same name or different names of different species of Hericium erinaceus, damage Issues such as the interests of breeders and producers, to achieve the effect of short detection time, high accuracy and reduced deviation

Inactive Publication Date: 2011-03-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] my country is rich in edible fungus resources and there are many varieties, but due to intentional or unintentional name changes by some producers, there is a confusion of "different species with the same name or the same species with different names" in the cultivated strains of Hericium erinaceus
This phenomenon greatly damages the interests of breeders and producers, not only brings obstacles to scientific research and academic exchanges, but also brings great difficulties to the formulation of standardized production and product quality standards

Method used

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  • Method for identifying hericium erinaceus strain king by utilizing molecule marker technology
  • Method for identifying hericium erinaceus strain king by utilizing molecule marker technology
  • Method for identifying hericium erinaceus strain king by utilizing molecule marker technology

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Embodiment 1

[0025] Embodiment 1: A kind of method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King

[0026] A method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King, comprising the following steps:

[0027] 1. Mycelia culture and collection.

[0028] (1) If the storage time of the test Hericium erinaceus strain is less than 6 months, the mycelia culture shall be carried out as follows:

[0029] 1. Use an inoculation rake to crush the PDA medium containing Hericium erinaceus mycelia, and transfer it to a 250 ml Erlenmeyer flask containing 100 ml PDA liquid medium;

[0030] 2. Place on a shaker at 25°C with a rotation speed of 90-130 r / min and incubate for 7-10 days;

[0031] 3. Filter the cultured mycelia with gauze, rinse with distilled water, and dry with filter paper;

[0032] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0033] (2) If the Hericium erina...

Embodiment 2

[0062] Embodiment 2: A kind of method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King

[0063] A method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King, comprising the following steps:

[0064] One, mycelia culture and collection, the specific method is the same as the step one of embodiment 1;

[0065] Two, the extraction of genomic DNA, described genomic DNA can be extracted from the mycelium of bacterial strain or fruit body; Concrete method is identical with the step 2 of embodiment 1;

[0066] 3. Establishment of the detection of SCAR-PCR molecular markers, wherein the SCAR-PCR amplification system is the same as the amplification system in Step 3 of Example 1; the SCAR-PCR reaction conditions are: 94° C. for 1 min;

[0067] 94°C 45second, 67°C 45second, 72°C 1min, 30 cycles; 72°C 5min.

[0068] The special detection primer Monkey King F2 / R2 uses PCR technology to obtain the specific DNA fra...

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Abstract

The invention discloses a method for identifying a hericium erinaceus strain king by utilizing molecule marker technology, which comprises the following steps of: the culture and collection of hyphae, the extraction of genome DNA, the detection establishment of sequence characterized amplified region-polymerase chain amplification (SCAR-PCR) molecular markers and the detection of an SCAR-PCR product. A detection primer king F2 / R2 is adopted, and a specific DNA band with the molecular weight of 37bp in the detection result of the SCAR-PCR product displayed in a DNA map is the marker of the hericium erinaceus strain king.

Description

technical field [0001] The invention relates to a method for identifying microorganisms, in particular to a method for identifying Hericium erinaceus strain Monkey King by using molecular marker technology, and belongs to the field of biotechnology. Background technique [0002] Hericium erinaceus ( Hericium erinaceus ) The meat is tender, delicious and nutritious. A long time ago, people listed it as one of the "Four Famous Dishes" together with bear's paw, sea cucumber, and shark's fin. Hericium erinaceus contains 9 kinds of amino acids necessary for human body. According to reports, the nutrients contained in Hericium erinaceus rank first and second compared with other edible fungus species cultivated artificially at present, so they seem more precious. [0003] my country is rich in edible fungus resources and there are many varieties, but due to intentional or unintentional name changes by some producers, there is a confusion of "different species with the same name ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 邓优锦谢宝贵江玉姬刘新锐温志强
Owner FUJIAN AGRI & FORESTRY UNIV
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