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New molecular marker for diagnosis and prediction of gastrointestinal tumor

A tumor and gastrointestinal technology, applied in the fields of biotechnology and medicine, can solve the problems of difficult early detection of gastrointestinal tumors

Active Publication Date: 2013-09-25
JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a detection method for predicting and providing information for diagnosing gastrointestinal tumors, which solves the difficult problem of early detection of gastrointestinal tumors in the prior art

Method used

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  • New molecular marker for diagnosis and prediction of gastrointestinal tumor
  • New molecular marker for diagnosis and prediction of gastrointestinal tumor
  • New molecular marker for diagnosis and prediction of gastrointestinal tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1. Differences in the expression of CST1 in different tissues of the human body

[0117] The human tissue samples used were purchased from commercial institutions except for the normal gastric mucosa and colonic mucosa which were collected by the inventor from the cooperative hospital. The nucleotide chip HG-U95Av of Affymetrix was used to compare the expression of CST1 mRNA in various normal tissues, and the operation process was referred to the product manual. The value of relative expression in this experiment is the normalized signal value after normalization by the fluorescence value of the housekeeping gene β-actin.

[0118] The result is as figure 2 As shown, except for the high expression of CST1 in the salivary gland, CST1 is not expressed in other tissues, indicating that the background value of CST1 mRNA expression in normal tissues is very low, which is extremely beneficial for the detection of CST1 under pathological conditions. It is overexpress...

Embodiment 2

[0119] Example 2. Comparison of CST1, CST1, 2, 4, and CST2 mRNA expression levels in gastric cancer and adjacent surgical tissue samples

[0120] By comparing the expression levels of CST1, CST1, 2, 4, and CST2 mRNA in 20 pairs (G1-G20) of gastric cancer and adjacent tissues, it was found that the expression of CST1 mRNA was the largest in gastric cancer and adjacent tissues. The results are shown in image 3 . All the above specimens passed the pathological proof, and real-time quantitative PCR was adopted by the dye method.

Embodiment 3

[0121] Example 3. Differences in the expression levels of the first splice mode (splice1) and the second splice mode (splice2) of CST1 in gastric cancer and paracancerous tissue samples

[0122] RNA extraction and reverse transcription of cDNA were carried out on the samples collected through surgery after pathological verification. Real-time quantitative PCR was used to detect the expression of the housekeeping gene ACTB in the samples, and it was found that the CP values ​​in the ACTB samples were basically the same. Figure 4a, that is to say, the total amount of cDNA in the sample is basically the same, and then compared the CP of the first splice mode (splice1) and the second splice mode (splice2) of CST1 in 20 pairs of gastric cancer and paracancerous tissues The median of the difference between the value and cancer, see Figure 4b , found that the difference in the expression of the first splice mode of CST1 (splice1) between cancer and para-cancer was significantly bet...

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Abstract

The invention discloses a detection method for predicting and diagnosing gastrointestinal tumor as well as a kit and a gene chip thereof. The method comprises the following steps: (1) obtaining a sample to be detected; and (2) detecting whether the following coding mRNA gene is overexpressed in the sample, wherein the gene is a CST1 gene shown in SEQ ID No:39, or a primer of one of mRNA of specificity recognition CST1 gene, CST1 cutter shown in SEQ ID No:43, SEQ ID No:44, cDNA of CST1 gene and cDNA of CST1 cutter, or / and a probe recognized gene, or a gene identified by using an amplicon corresponding to at least one CST1 primer. The method can be applied to diagnosis of gastrointestinal caner, dynamic monitoring and prognosis judgment and has the advantages that a large number of verification samples can be obtained, the result is accurate and reliable result, the sensitivity is high and the like.

Description

technical field [0001] The present invention belongs to the fields of biotechnology and medicine, and more specifically, the present invention relates to a gastrointestinal cancer marker and its application, a reagent or kit for the diagnosis, dynamic detection and prognosis judgment of gastrointestinal cancer and its use method. Background technique [0002] In recent years, the incidence of malignant tumors has continued to rise, and more than 60% of them are digestive system tumors, with gastric cancer and colorectal cancer being the most common. Globally, the incidence of gastric cancer is second only to lung cancer among male malignancies, and ranks fourth among female malignancies. In my country, the death rate ranks first among malignant tumors, with an average annual death rate of 25.53 per 100,000 population. Colorectal cancer, including colon cancer and rectal cancer, is one of the fastest-rising tumors in most countries and regions in the world in terms of incide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 王弢陈菲渠香云
Owner JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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