Radix trichosanthis saponin and application of radix trichosanthis saponin in preparing medicine for treating ischemic cerebrovascular diseases

Abstract

The invention discloses a radix trichosanthis saponin and application of radix trichosanthis saponin in preparing a medicine for treating ischemic cerebrovascular diseases. The extraction method of the radix trichosanthis saponin is as follows: a raw material radix trichosanthis is smashed and then water is added in the radix trichosanthis for ultrasonic extraction; the obtained extracting solution is extracted after extracting; the supernatant is separated and purified by macroporous absorption resins after extracting; further purification is carried out on the supernatant by adopting a silicagel column chromatography; elution is carried out by adopting water after purification to be in a transparent state; the elution is carried out by utilizing ethanol; and the collected ethanol eluentis successively subject to rotary evaporation drying vacuum drying in order to obtain the radix trichosanthis saponin. The radix trichosanthis saponin has the action of obviously prolonging the breath time and the breath times of a mouse after decollation, reducing cerebral edema, enhancing the survival rate and prolonging the survival time of an animal with cerebral ischemia, reducing the cerebral infarction area, restraining the formation of thrombosis in vivo and the like, and the toxin or side effect is less. Therefore, the obtained radix trichosanthis saponin of the invention has good treatment action on the ischemic cerebrovascular diseases.

Description

technical field [0001] The present invention relates to a method for extracting trichosanthin and related uses thereof, in particular to a trichosanthin saponin and its use in the preparation of medicines for treating ischemic cerebrovascular diseases. Background technique [0002] Cerebrovascular disease is one of the three major diseases that lead to human death. Globally, about 4.6 million people die every year. At the same time, it has the characteristics of high morbidity and disability. Cerebrovascular diseases are divided into ischemic and hemorrhagic cerebrovascular diseases. The former is more common clinically, accounting for 70-80% of all cerebrovascular patients. Due to cerebral arteriosclerosis and other reasons, the cerebral artery lumen is narrowed, the blood flow is reduced or completely blocked, the blood circulation in the brain is disturbed, and a series of symptoms occur due to brain tissue damage. The latter is mostly caused by long-term hypertension, ...

AI Technical Summary

Problems solved by technology

The systematic research on trichosanthum found that trichosanthin saponin is the effective part of trichosanthum pollen, but so far there is no relevant report on the application of trichosanthin saponin in the preparation of drugs for the treatment of cerebral ischemia, cerebral ischemia / reperfusion, cerebral infarction and other cerebrovascular diseases

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