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Method for producing functional soy peptide by alcohol leaching soy protein concentrate

A technology of concentrated soybean protein and soybean polypeptide, which is applied in the direction of protein food processing, plant protein processing, protein food ingredients, etc., can solve the problems that it is difficult to increase the total nitrogen content of the product, increase the difficulty of increasing soybean polypeptide, and it is difficult to deeply hydrolyze, etc., to achieve The effect of reducing the possibility of product contamination, shortening the processing time, and increasing the content of soluble nitrogen

Inactive Publication Date: 2011-03-02
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, it should be noted that most of the protein in the soybean protein concentrate produced by the alcohol method has been denatured, the solubility is poor, and it is difficult to carry out deep hydrolysis. The content of total nitrogen, the existence of these problems has increased the difficulty of producing soybean peptides using alcohol-based soybean protein concentrates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for producing functional soybean polypeptides using alcohol-process soybean protein concentrate, the specific implementation steps are as follows:

[0032] ((1) Raw material liquid preparation

[0033] Take 1 part of alcohol-processed soybean protein concentrate and 6-12 parts of distilled water according to the weight ratio, and mix them to form a uniform soybean protein concentrate slurry;

[0034] (2) Cellulase hydrolysis pretreatment

[0035] Use hydrochloric acid solution with a weight content of 1-20% to adjust the pH value of the soybean protein concentrate slurry to 4-6, add cellulase, the amount of enzyme added is 0.5%-2% of the weight of the alcohol-process soybean protein concentrate raw material, stir evenly, Enzymolysis at 40-50°C for 2-3 hours; centrifuge the cellulase treatment solution at 2000-5000 rpm for 5-10 minutes, remove the supernatant, and add the same volume of distilled water as the supernatant , stirred to become the primary pretrea...

Embodiment 2

[0053] (1) Preparation of raw material solution

[0054] Take 1kg of alcohol-processed soybean protein concentrate from Tianjin Shifa Guanhua Biotechnology Co., Ltd. and mix it with 9kg of distilled water, and stir to form a uniform soybean protein concentrate slurry;

[0055] (2) Cellulase hydrolysis pretreatment

[0056] Use hydrochloric acid solution with a weight content of 10% to adjust the pH value of the soybean protein concentrate slurry to 5.0, add cellulase, the amount of enzyme added is 1.5% of the weight of the alcohol-process soybean protein concentrate raw material, stir evenly, and enzymatically hydrolyze at 45°C 2 hours; centrifuge the cellulase treatment solution at 2000 rpm for 10 minutes, remove the supernatant, and add distilled water of the same volume as the supernatant, and stir to become the primary pretreatment solution of soybean protein concentrate;

[0057] (3) Ultra-high temperature transient pretreatment

[0058] The soybean protein concentrate ...

Embodiment 3

[0070] (1) Preparation of raw material solution

[0071] Take 1kg of Soybean Milk from Yihai (Fangchenggang) Soybean Industry Co., Ltd. and mix it with 10kg of distilled water to form a uniform soybean protein concentrate slurry;

[0072] (2) Cellulase hydrolysis pretreatment

[0073] Use 10% hydrochloric acid solution by weight to adjust the pH value of the concentrated soybean protein slurry to 5.0, add cellulase, the amount of enzyme added is 2% of the weight of the raw material of concentrated soybean protein by alcohol method, stir evenly, and enzymatically hydrolyze at 50°C 3 hours; centrifuge the cellulase treatment solution at 5000 rpm for 6 minutes, remove the supernatant, and add distilled water of the same volume as the supernatant, and stir to become the primary pretreatment solution of soybean protein concentrate;

[0074] (3) Ultra-high temperature transient pretreatment

[0075] The soybean protein concentrate primary pretreatment liquid is subjected to ultra-...

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Abstract

The invention relates to a method for producing functional soy peptide by alcohol leaching soy protein concentrate, which prepares soy peptide by a two-stage protease hydrolysis process. The method comprises the following main steps: hydrolyzing and preprocessing feed solution cellulose; carrying out instantaneous pretreatment at ultra temperature; hydrolyzing protease; carrying out pH regulation and centrifugal separation; carrying out ultrafiltration separation; and condensing and drying. In the process, the raw material of alcohol leaching soy protein concentrate is performed with cellulose hydrolysis pretreatment and instantaneous pretreatment at ultra temperature, and overcomes the deficiencies of poor dissolubility of alcohol leaching soy protein concentrate and low total nitrogen content of the product; enzymolysis liquid pH value is regulated twice to precipitate protein, and two-stage enzymolysis process control is carried out; and while saving energy, the produced soy peptide product has good taste and high quality. The invention provides a new way for the deep processing of the alcohol leaching soy protein concentrate. The product of the invention has obvious practical effect and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of deep processing of soybean products and relates to a method for producing soybean polypeptide, in particular to a method for producing functional soybean polypeptide by utilizing concentrated soybean protein by alcohol method. Background technique [0002] With the continuous development of food science and technology, the development of soybean deep-processing products is gradually deepening. The production of soybean polypeptide by soybean protein hydrolysis is an important direction of soybean deep processing. Soy polypeptide not only has good nutritional properties, but also has a wide range of physiological and pharmacological effects and more functional properties than soybean protein. It is a promising functional food raw material. The research and development of soybean polypeptide has become a hotspot in the emerging high-tech biological field in the world, and has great market potential. [0003] Both so...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/16
Inventor 许晓菁何雨青王祥河
Owner 天津实发中科百奥工业生物技术有限公司
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