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Novel quasi-physiological pulsating flow environment arterial blood vessel tissue engineering reactor

A technology for arterial blood vessels and tissue engineering, applied in the fields of cell culture and tissue engineering, which can solve the problems of high motor power and other parameters, complex structures, etc.

Active Publication Date: 2011-02-16
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reactor not only has a relatively complex structure, but also has high requirements on parameters such as motor power, so it is not suitable to use a single pulse source to provide pulse drive for multiple culture circuits.

Method used

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  • Novel quasi-physiological pulsating flow environment arterial blood vessel tissue engineering reactor
  • Novel quasi-physiological pulsating flow environment arterial blood vessel tissue engineering reactor
  • Novel quasi-physiological pulsating flow environment arterial blood vessel tissue engineering reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. According to figure 2 Connect the reactor components, including a liquid storage bottle 27, a liquid driver 21, a compliance regulator 22, a first resistance regulator 23, a pulse generator 24, a dynamic culture chamber 25 for vascular tissue, and a second resistance regulator 26;

[0069] 2. Disinfection reactor, disinfection conditions: 130°C, 3 atmospheres, 1 hour;

[0070] 3. Install the tissue engineering vascular culture in the vascular tissue dynamic culture chamber 25;

[0071] 4. Prepare the medium according to the cultivation requirements, and inject the sterile medium into the storage bottle;

[0072] 5. Set the flow rate of the liquid driver, set the motion frequency of the linear stepper motor to 70 times per minute, and set the initial position between the motor gain and 1-5%; start the liquid driver and the linear stepper motor to start perfusion culture;

[0073] 6. Adjust resistance regulators 23, 26 and compliance regulator 22 to control the medi...

Embodiment 2

[0075] 1. According to figure 2 Connect the reactor components, including a liquid storage bottle 27, a liquid driver 21, a compliance regulator 22, a first resistance regulator 23, a pulse generator 24, a dynamic culture chamber 25 for vascular tissue, and a second resistance regulator 26;

[0076] 2. Disinfection reactor, disinfection conditions: 130°C, 3 atmospheres, 1 hour;

[0077] 3. Install tissue engineered vascular cultures in the vascular tissue dynamic culture chamber;

[0078] 4. Prepare the medium according to the cultivation requirements, and inject the sterile medium into the storage bottle;

[0079] 5. Set the flow rate of the liquid driver 21, set the motion frequency of the linear stepper motor to 70 times / min, and set the initial position between the motor gain 1-5%; start the liquid driver and the linear stepper motor, and start perfusion culture ;

[0080] 6. Adjust the resistance regulators 23, 26 and compliance regulators to control the pressure and fl...

Embodiment 3

[0083] 1. According to Figure 5 Connect the reactor components, including a liquid storage bottle 57, a liquid driver 51, a compliance regulator 52, a first resistance regulator 53, a pulse generator 54, a heart valve test cavity 55, and a second resistance regulator 56;

[0084] 2. Install the heart valve 58 to be tested in the heart valve experiment chamber;

[0085] 3. Fill the buffer into the storage bottle;

[0086] 4. Set the flow rate of the liquid driver 21, set the motion frequency of the linear stepper motor to 70 times per minute, and set the motor gain between 1-5%, set the initial position; start the liquid driver and the linear stepper motor, and start the perfusion test ;

[0087] 5. Adjust the resistance regulators 53, 56 and compliance regulators to control the pressure and flow waveforms flowing through the heart valve test cavity 55: the inlet pressure is between 110-140mmHg, and the outlet pressure is between 85-110mmHg.

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PUM

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Abstract

The invention relates to a novel quasi-physiological pulsating flow environment arterial blood vessel tissue engineering reactor. The reactor comprises a liquid storage device 27, a liquid driver 21 and a blood vessel tissue dynamic culture chamber 25 which are sequentially connected through a fluid pipeline to form a fluid circulation loop, and a pulsation generator 24, wherein the liquid driver21 drives a liquid culture medium to circularly flow; the pulsation generator 24 forms the pulsation of the circularly flowing liquid culture medium and comprises a pulsation chamber 241 which is a closed chamber with fixed volume, a closed piston 244 in the pulsation chamber 241, a linear steeping motor 243 and a pull rod 242; the pull rod connects the closed piston 244 with a driving shaft of the linear stepping motor 243; and the closed chamber is communicated with the fluid pipeline above the culture chamber 25.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more particularly relates to a vascular tissue engineering reactor. Background technique [0002] In the field of tissue engineering, tissue engineering bioreactor is an important technical means to study the influence of different environmental factors (physical and biochemical factors) on the three-dimensional functional culture of specific cells and tissues. Automation, online monitoring, etc.), making tissue engineering a key link in the process of entering standard industrial scale production and clinical application from the laboratory. Therefore, targeted research and development of advanced bioreactors for tissue engineering is of great significance to the development of tissue engineering. [0003] So far, autograft, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels cannot become ideal arterial blood vessel substitutes (...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 樊瑜波邹远文贡向辉李晋川黄学晋
Owner BEIHANG UNIV
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