Herbal composition for osteoarthritis
A technology of herbal medicine and agarwood, which can be used in anti-inflammatory agents, bone diseases, medical formulas, etc., and can solve problems such as uncertain effectiveness
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[0021] 1500 grams of lettuce, 150 grams of agarwood, 600 grams of rice, 150 grams of frankincense, 75 grams of Atractylodes macrocephala, and 150 grams of dodder were heated in 3 liters of deionized water at 100±5°C for 2.5 hours. The extract was collected. Repeat again. The secondary extracts were combined and passed through a 100-mesh sieve. The extract is concentrated and then sprayed and dried into powder (SO101C).
[0022] Experiment 1: Stimulation of Chondrocyte Proliferation
[0023] Human cartilage was obtained from joint replacement surgery with the informed consent of the patient. The cartilage was treated with bacterial secondary / quaternary collagenase (Collagenase, Worthington Chemical, Freehold, NJ) 1 mg / ml DMEM+10% fetal calf serum (FCS)+25 μg / ml Gentamicin (Gentamicin, Grand Island, NY) (C-DMEM) was digested overnight at 37°C, and the obtained cells were cultured in C-DMEM with 5% carbon dioxide at 37°C at high density.
[0024] 1×10 per hole 4 The cells we...
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