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Rhodopseudomonas spheroides strain and liquid inoculant thereof as well as preparation method and application thereof

A technology of Rhodopseudomonas and liquid inoculum, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve the problem of high cost, achieve low production cost, reduce pesticide residues, The effect of protecting the ecological environment

Active Publication Date: 2012-05-09
CHANGSHA AGREEN BIO TECH LTD CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Laboratory and practical application studies have shown that the use of microorganisms to eliminate chemical pesticide residues is environmentally friendly, practical, and easy to operate. It has become an important method for removing pesticide residue pollution, but the cost of this method is relatively high. The removal effect needs to be further strengthened

Method used

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  • Rhodopseudomonas spheroides strain and liquid inoculant thereof as well as preparation method and application thereof
  • Rhodopseudomonas spheroides strain and liquid inoculant thereof as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0021] This example investigates the degradation of high-concentration pyrazosulfuron-methyl by the Rhodopseudomonas strain CCTCC M 2010144 of the present invention in a liquid medium.

[0022] First, pyrazosulfuron-methyl original drug is dissolved in acetone, and the dissolved pyrazosulfuron-methyl acetone solution is sterilized by filtering through a 0.22 μm filter membrane, and added to the sterilized seed liquid culture medium of the present invention until pyrazosulfuron-methyl The final concentration is 200mg / L. The seed liquid medium used was formulated from the following components: (NH 4 ) 2 SO 4 1g~2g, MgSO 4 ·7H 2 O 0.2g~0.5g, NaHCO 3 5g~6g, K 2 HPO 40.5g~1g, peptone 2g~2.5g, sodium acetate 2g~3g, NaCl 0.2g~0.5g and yeast extract 1.5g~1.7g, then dilute to 1000mL with distilled water, control the pH value to 7.0~7.2, after 121 It can be obtained after sterilization at ℃ for 30 minutes. In the above-mentioned seed liquid culture medium containing pyrazosul...

Embodiment 2

[0028] This example investigates the degradation of pyrazosulfuron-methyl by the liquid bacterial agent of Rhodopseudomonas strain CCTCC M 2010144 of the present invention in soil.

[0029] Weigh 100 g of sterilized paddy field soil, add pyrazosulfuron-methyl, so that the drug concentration in the soil is 20 mg / kg.

[0030] Adopt the following method to prepare a kind of liquid bacterial agent of rhodopseudomonas bacterial strain of the present invention:

[0031] (1) Activation: The Rhodopseudomonas strain CCTCC M 2010144 (containing 20% ​​glycerol) obtained by screening and stored at -80°C was inoculated on a double-layer sandwich solid medium plate for activation. The solid medium used contained the following Concentration of components: (NH 4 ) 2 SO 4 0.1g / mL, MgSO 4 0.02g / mL, NaHCO 3 0.5g / mL, K 2 HPO 4 0.05g / mL, NaCl 0.02g / mL, yeast extract 0.15g / mL, agar 1.8g / mL; control the pH value of the solid medium to 7.0-7.2, control the activation temperature at 30°C-35...

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Abstract

The invention particularly discloses a rhodopseudomonas spheroides strain and a liquid inoculant thereof as well as a preparation method thereof and application thereof. A preservation name of the rhodopseudomonas spheroides strain is rhodopseudomonas spheroides S8-1, and a preserving number is CCTCC M 2010144. In the liquid inoculant, the thalli concentration of the rhodopseudomonas spheroides S8-1 reaches above 1bn. / ml. The preparation method of the liquid inoculant comprises the following steps of inoculating the rhodopseudomonas spheroides S8-1 into a double-layer sandwich solid culture medium flat plate to be activated; then inoculating into seed liquid culture medium to carry out seed culture and fermentation culture; and controlling the temperature at 30-35 DEG C and the illumination intensity at 4500-7500 lux in the activation and culture process, and continuing the fermentation culture till a logarithmic growth phase to prepare the liquid inoculant. The strain and the liquid inoculant can efficiently degrade herbicide pyrazosulfuron-ethyl residues in soils to solve the safety problem of succeeding crops endangered by the chemical herbicide residues in the soils.

Description

technical field [0001] The invention belongs to the technical field of utilizing biodegradable chemical pesticide residues, and specifically relates to a rhodopseudomonas and its bacterial agent, as well as a corresponding preparation method and application. Background technique [0002] The use of chemical herbicides in chemical pesticides has restored a large number of losses caused by weed damage in agricultural production, and at the same time greatly improved the labor efficiency and mechanization of agricultural production, and played an inestimable role in the modernization of agricultural production. . However, the chemical herbicides widely used in agricultural production, its residues in the environment not only seriously pollute the environment, but also cause excessive chemical herbicide residues in agricultural products, seriously affecting the quality and quality of agricultural products; moreover, chemical herbicides Herbicide selectivity is strong, and the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/04A62D101/28C02F101/30
Inventor 刘勇尹乐斌张德咏张松柏罗香文成飞雪罗源华朱春晖张驰
Owner CHANGSHA AGREEN BIO TECH LTD CO
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