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Nucleotide carrying modifiers and preparation method thereof and method for gene sequencing

A technology for gene sequencing and modifiers, applied in the field of genetic engineering, can solve the problems of short sequencing length and low sequencing accuracy, and achieve the effect of improving accuracy and long gene sequences

Inactive Publication Date: 2011-01-12
SHENGZHEN CHINA GENE TECH COMPANY
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Problems solved by technology

[0006] One of the purposes of the present invention is to provide a modified nucleotide, which aims to solve the problems of low sequencing accuracy and short sequencing length in the prior art

Method used

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  • Nucleotide carrying modifiers and preparation method thereof and method for gene sequencing
  • Nucleotide carrying modifiers and preparation method thereof and method for gene sequencing
  • Nucleotide carrying modifiers and preparation method thereof and method for gene sequencing

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Embodiment Construction

[0064] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0065] The general structural formula of the modified nucleotide in the present invention is: dNTP-R. In the formula, dNTP is a nucleotide, and the modifier-R is connected to a position in the nucleotide other than -OH on the deoxyribose, which includes at least one excisable site and at least one label. in:

[0066] (1) Nucleotide dNTP includes four types: dATP, dGTP, dCTP, and dUTP, all of which are composed of a nitrogenous base, a deoxyribose, and a phosphate. For example, the molecular structure of one of the nucleotides, dATP, is as follows:

[0067]

[0068] For dUTP, dTTP may also be used instead, and the embodiments of the present invention are all described with dUTP, which is only for the purpose of concise description, rather than ...

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Abstract

The invention relates to the gene engineering field, specifically to a nucleotide carrying modifiers and a preparation method thereof and a method for gene sequencing. The structural general formula of the nucleotide carrying modifiers is dNTP-R; in the formula, the dNTP is nucleotide; the modifier -R is connected on a position in the nucleotide except from -OH on a deoxyribose and comprising at least a cutting site and at least a marker. The preparation method thereof comprises the following steps: the position except from the -OH on a deoxyribose is connected with the nucleotide of a first modifier -R1 to react with a second modifier -R2 with the cutting site to combine the nucleotide dNTP-R carrying the modifier. Furthermore, the invention provides a method for gene sequencing using the nucleotide carrying modifiers. When the nucleotide carrying modifiers provided by the invention carries out the gene sequencing, the sequencing accuracy rate is improved and the longer gene sequence is measured.

Description

technical field [0001] The invention relates to the field of genetic engineering, more specifically, to a modified nucleotide, a preparation method thereof, and a method for gene sequencing. Background technique [0002] In traditional gene sequencing technology, the dideoxy chain termination method (Sanger) is used. Add nucleotides to the reaction system for DNA synthesis reaction, the bases on the nucleotides gradually bind to different positions of the DNA template to be tested, at the end of a synthesis, because each base stops at a different position, resulting in the generation of If the DNA double strands are different in length, electrophoresis is used to separate them, and then ATCG is marked, and each base is determined according to different stop positions. [0003] A current sequencing method is as follows: first, the template is hybridized with the primer; then DNA polymerase and modified nucleotides are added, and the 3-terminal -OH of the nucleotides is conne...

Claims

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Application Information

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IPC IPC(8): C07H19/20C07H19/10C07H1/00C09K11/06C12Q1/68G01N21/64
Inventor 盛司潼
Owner SHENGZHEN CHINA GENE TECH COMPANY
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