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Molecular marking method of soybean low phosphorus-resistant gene GmAPt

A technology of molecular markers and low phosphorus tolerance, applied in the field of molecular genetics, can solve the problem of low accuracy, achieve the effect of heavy workload, broaden the genetic background, and strong stress tolerance

Inactive Publication Date: 2010-12-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] In recent years, with the development of molecular marker technology, many researchers have used markers linked to phosphorus efficiency QTLs for assisted selection of low phosphorus-tolerant germplasm. This method improves the speed of selection to a certain extent and is easy to operate. , but these markers are linked markers of soybean phosphorus efficiency genes, so the accuracy is generally low

Method used

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  • Molecular marking method of soybean low phosphorus-resistant gene GmAPt
  • Molecular marking method of soybean low phosphorus-resistant gene GmAPt
  • Molecular marking method of soybean low phosphorus-resistant gene GmAPt

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Experimental program
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Embodiment Construction

[0021] (1) Test materials

[0022] The test materials included 14 materials (Table 1, all provided by the National Soybean Improvement Center) from soybean low-phosphorus-tolerant germplasm reported in different literatures (Liu Ying et al., 2007; Cui Shiyou et al., 2007; Ding Yuchuan et al., 2006) , including 6 low-phosphorus-tolerant germplasms, 7 low-phosphorus-intolerant germplasms and 1 medium-tolerant germplasm.

[0023] Table 1 14 different materials related to low phosphorus resistance

[0024]

[0025] (2) Acquisition of Indel S170 mark

[0026] According to a 10bp deletion site inside the GmAPt gene sequence, a pair of Indel marker primers were designed, the upstream primer was 5'-CTTGAGATCACTACAACACACC-3', and the downstream primer was 5'-GATAGAAGAAC AACACAAAAAC-3'

[0027] (3) Extraction of DNA

[0028] Using fresh leaves at the seedling stage as materials, DNA was extracted by the CTAB method. The detailed steps are as follows:

[0029] 1) Take about 3-5 gr...

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Abstract

The invention relates to a molecular marking method of a soybean low phosphorus-resistant gene GmAPt, belonging to the field of molecular genetics. A marker Indel_S170 is designed and synthesized by utilizing 10bp deletion existing in the first introns of the soybean low phosphorus-resistant gene GmAPt in a soybean low phosphorus-resistant material and a low phosphorus-nonresistant material, and because the marker is the self marker of the gene, the marker expresses coseparation with the low phosphorus-resistant gene GmAPt and allelic genes thereof. By utilizing the marker, the low-phosphorus resistance of a soybean germ-plasm resource can be accurately and rapidly identified, the selecting efficiency and the split-type identifying efficiency of the soybean low phosphorus-resistant material are greatly improved, and the auxiliary selecting and breeding progress of a soybean low phosphorus-resistant molecular marking is quickened.

Description

1. Technical field [0001] The invention provides a molecular marker method of soybean low phosphorus tolerance gene GmAPt, which belongs to the field of molecular genetics and can be used for soybean low phosphorus tolerance molecular marker assisted selection breeding and germplasm typing identification. 2. Background technology [0002] Soybean is an important food crop and oil crop in my country. However, phosphorus deficiency in two-thirds of the arable land is the main factor limiting soybean production in my country. Therefore, exploring the potential of soybean itself and efficiently utilizing the potential phosphorus resources in the soil is an effective way to solve the phosphorus deficiency of soybean. [0003] Although some progress has been made in the study of soybean phosphorus efficiency, there are still great difficulties in the screening of phosphorus efficient genotypes, mainly because of the difficulty in determining the screening index and screening peri...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 喻德跃张丹程浩
Owner NANJING AGRICULTURAL UNIVERSITY
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