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Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis

A technology of Bacillus aureus and Avermectin, which is applied in the directions of insecticides, biocides, animal repellents, etc., can solve the problems of unsatisfactory compound effect of Bacillus thuringiensis and Avermectin, and achieve the goal of delaying pests. The effect of drug resistance, long validity period and fast onset

Inactive Publication Date: 2010-12-22
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the unsatisfactory effect of direct mixing and compounding of Bacillus thuringiensis and Avi powder in the prior art, the present invention provides a biological suspension insecticide compounded with abamectin and Bacillus thuringiensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of 2% Avi and 2700IU Bt Compound Suspending Concentrate

[0024] The mass ratio of each component is: 90% of Bacillus thuringiensis fermentation broth with a toxicity titer of 3000IU / mg, 2.2% of abamectin with a purity of 95%, and the auxiliary agent is the dispersant polyoxyalkane produced by bamboo oil and fat 14% ether FS, 1% urea, 1.2% magnesium aluminum silicate, 1.4% organic bentonite, 0.1% xanthan gum, and 0.1% butyl-p-cresol, all of which are common chemical raw materials.

[0025] Preparation method: According to the above formula, weigh an appropriate amount of Bacillus thuringiensis fermentation broth, avermectin, urea, magnesium aluminum silicate, organic bentonite and swollen xanthan gum and add them to the ball mill reaction kettle together, stir at a low speed for 30 minutes, Add polyoxyalkylene ether FS1, stir at high speed for 2 hours, then add tricrene butyl-p-cresol and stir evenly to obtain a compound biosuspension insecticide of avermect...

Embodiment 2

[0035] Preparation of 3% Avi·2500IU Bt Compound Suspending Concentrate

[0036] The mass ratio of each component is: 85% of Bacillus thuringiensis fermentation broth with a toxicity titer of 3000IU / mg, 3.2% of abamectin with a purity of 95%, auxiliary agent polyoxyalkylene ether FS14.5%, urea 1%, magnesium aluminum silicate 1.4%, organic bentonite 1.5%, xanthan gum 0.1%, terrene butyl p-cresol 0.1%, benzoate 3.2%.

[0037] Preparation method: according to the above formula, weigh an appropriate amount of Bacillus thuringiensis fermentation broth, abamectin, urea, magnesium aluminum silicate, benzoate, organic bentonite and swollen xanthan gum and add them to the ball mill reaction kettle together, stirring at a low speed After 30 minutes, add polyoxyalkylene ether FS1, stir at a high speed for 2 hours, then add tricretyl p-cresol and stir evenly to obtain a biosuspension insecticide compounded with abamectin and Bacillus thuringiensis.

[0038] After the preparation is comple...

Embodiment 3

[0047] Suspension prepared by embodiment 1 is to the indoor toxicity determination of cotton bollworm

[0048] In the indoor toxicity test, the 3rd instar cotton bollworm was used as the test insect, and the toxicity test was carried out by dipping method, repeated 4 times, and the effect of 4 days after the drug was investigated, the corrected mortality was calculated, and the logarithm of the drug concentration---death The regression equation of the probability value, and the chi-square suitability test, and then calculate the LC50, and calculate the relative toxicity index (TI) and the co-toxicity coefficient (CTC) of the mixture by referring to Sun Yunpei's method. The test results are shown in Table 1. When Bacillus thuringiensis is mixed with abamectin, there is a synergistic effect.

[0049] Table 1 Bacillus thuringiensis abamectin is to the indoor toxicity determination of cotton bollworm (4 days after the medicine)

[0050] deal with

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PUM

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Abstract

The invention relates to a biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis, which comprises 88-93 percent by weight of main agent and 7-12 percent by weight of auxiliary agent, wherein the main agent is a mixture of the abamectin and the bacillus thuringiensis; the auxiliary agent comprises a dispersing agent, a thickening agent and other assistants; and the dispersing agent is polyoxyalkylene ether FS1 and urea and magnesium aluminum silicate. According to the formula, the biological suspension pesticide is prepared by the steps of: adding a bacillus thuringiensis fermentation liquor, the abamectin, the urea, the magnesium aluminum silicate and the thickening agent into a ball-milling reactive kettle, stirring at low speed, adding the FS1, and stirring at high speed; and then adding the other assistants for stirring uniformly to obtain the biological suspension pesticide. In the biological suspension pesticide, the content of the abamectin is not less than 2 percent, the content of insecticidal crystal protein of the bacillus thuringiensis is kept above 80 percent, the survival rate of the bacillus thuringiensis spore is above 90 percent, and the cotoxicity coefficient of the pesticide is more than 120. The invention is beneficial to delaying the generation of pesticide resistance of insects, and has the advantages of quick effect-taking, long valid period and low cost lower than or not more than that of the traditional similar pesticides.

Description

technical field [0001] The invention belongs to the technical field of a compound biological insecticide for killing lepidopteran pests, in particular to a suspension insecticide compounded by Bacillus thuringiensis and abamectin. technical background [0002] Lepidoptera pests are the main and important pests in agriculture. They harm crops in a wide range and in a large degree. The control of such pests has always been an important aspect of agricultural production. At present, there are chemical pesticides and biological pesticides commonly used at home and abroad, among which chemical pesticides take the main position, and biological pesticides account for about 2%, mainly including Bacillus thuringiensis, abamectin, and spinosad. [0003] Bacillus thuringiensis (Bt) is a Gram-positive Agrobacterium belonging to the genus Bacillus. The insecticidal protein and spores produced by it have high insecticidal activity and are used to control diamondback moth and cotton bollwo...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N43/90A01N25/04A01P7/04
Inventor 胡雪芹刘坤张洪斌
Owner HEFEI UNIV OF TECH
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