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Peptide-lipid constructs and their use in diagnostic and therapeutic applications

A constructive, reactive technique for peptide-lipid constructs for diagnostic and therapeutic use, the field of construction of methods

Inactive Publication Date: 2010-12-15
KODE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, PEG spacers with a high number of oxyethylene units may cause nonspecific adhesion of proteins (including antibodies in some individuals) and / or nonspecific activation of the complement cascade

Method used

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  • Peptide-lipid constructs and their use in diagnostic and therapeutic applications
  • Peptide-lipid constructs and their use in diagnostic and therapeutic applications
  • Peptide-lipid constructs and their use in diagnostic and therapeutic applications

Examples

Experimental program
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Embodiment Construction

[0228] In general, the present invention provides a structure (L-S-) i F(-S-L) j The peptide-lipid structure of which:

[0229] F is a peptide;

[0230] S is a spacer covalently linking F to L;

[0231] L is a lipid selected from the group consisting of diacyl-glycerides and dialkyl-glycerides, including glycerophospholipids;

[0232] i and j are each 0 or 1;

[0233] As well as the use of these peptide-lipid constructs in diagnostic and therapeutic applications.

[0234] When i is 0 and j is 1, the peptide-lipid construct has the structure:

[0235] F-S-L

[0236] When i is 1 and j is 0, the peptide-lipid construct has the structure:

[0237] L-S-F

[0238] When S is linked to F through the amino terminus of the peptide, this configuration is represented by the structure or substructure L-S-F.

[0239] When S is linked to F through the carboxyl terminus of the peptide, this configuration is represented by the structure or substructure F-S-L.

[0240] When S is linke...

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Abstract

Peptide-lipid constructs of the structure L-S-F are disclosed, where F is a peptide, S is a spacer covalently linking F to L via an oligomer of ethylene glycol, and L is a diacyl- or dialkyl-glycerolipid (including glycerophospholipids). The spacer ideally has 6 to 14 ethylene glycol repeats, corresponding to PEG with a molecular weight of approximately 250 to 600. Also disclosed is a method of detecting reactive antibodies in serum by contacting serum with cells modified to incorporate a peptide-lipid construct, where the peptide is an epitope of the antibody, and determining the degree of agglutination of the cells.

Description

technical field [0001] The present invention relates to methods for effecting qualitative and quantitative changes in the level of peptides expressed on the surface of cells and multicellular structures, and constructs for such methods. [0002] In particular, the invention relates to peptide-lipid constructs for diagnostic and therapeutic uses, including serodiagnosis. Background technique [0003] The ability to effect qualitative and quantitative changes in the level of peptides expressed on the surface of cells and multicellular structures has many diagnostic and therapeutic applications. [0004] Changes in the nature and quantity of surface-expressed peptide levels can alter transmembrane transport, cell-solute and cell-cell interactions, and thereby alter the function of the modified cell or multicellular structure. [0005] Known methods of causing such changes include genetic manipulation, chemical modification of endogenous membrane peptides, and "cell surface pai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/539C07K1/04G01N33/536A61K39/385C07K17/08G01N33/564C07F9/10G01N33/53G01N33/68C07K1/02G01N33/531G01N33/92
CPCG01N2405/00C07K5/1008C07K14/47C07F9/5721G01N33/554G01N33/80A61K47/48046C12N2503/00C12N5/0641G01N33/92C07F9/091G01N33/555A61K47/48053G01N33/6854C12N5/0006G01N33/5304C07K17/08A61K47/543A61K47/544C07F9/572
Inventor 克里斯蒂娜-西蒙娜·温伯格尼乔莱·博温斯蒂芬·迈克尔·亨利
Owner KODE BIOTECH
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