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Technical method for decoloring and deproteinizing corn stigma polysaccharide

A corn silk polysaccharide and deproteinization technology is applied in the field of refining and processing plant active ingredients, and can solve the problems of inability to remove apparent pigments, large amount of reagents, solvent residues and the like

Inactive Publication Date: 2010-12-15
JILIN INST OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sevag method emulsifies the protein, but chloroform is a second-class solvent and is a toxic substance, which is easy to cause solvent residues; the trichloroacetic acid method is easy to cause the degradation of polysaccharides, which is not suitable for application in industrial production; However, the apparent pigment cannot be removed; the enzymatic method for protein removal has mild operating conditions, no pollution, and high protein removal rate
Although the sevag method can achieve the purpose of removing protein and purifying polysaccharides, the sevag method requires a large amount of reagents and is highly toxic; I have used enzymatic methods to remove proteins from corn silk polysaccharides, and the effect is remarkable, and the loss rate of polysaccharides is low. The combined use of the two sevag reagents reduces the amount of reagents and may achieve better results

Method used

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  • Technical method for decoloring and deproteinizing corn stigma polysaccharide
  • Technical method for decoloring and deproteinizing corn stigma polysaccharide
  • Technical method for decoloring and deproteinizing corn stigma polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] bleaching

[0036] 1. Determination of absorbance of corn silk crude polysaccharide pigment

[0037] The crude polysaccharide solution was scanned at all wavelengths, and the results showed that the solution had no maximum absorption wavelength in the visible range. According to the principle of complementary colors, the color presented by the solution is the complementary color that it absorbs light. Since the polysaccharide solution is orange-yellow after dilution before and after decolorization, the solution mainly absorbs visible light in the blue band. Therefore, the 450nm wavelength at the center of the band is selected as the detection wavelength to measure the absorbance of the solution. Use distilled water as the blank solution, and measure the absorbance of the decolorized sample solution at a wavelength of 450nm. Polysaccharide decolorization rate (%)=(pigment absorbance before decolorization-pigment absorbance after decolorization) / pigment absorbance befor...

Embodiment 2

[0058] Example 2 Enzymatic protein removal

[0059] Table 5 Enzymatic factor level table

[0060]

[0061] Table 6 Enzymatic orthogonal test results

[0062]

[0063] From Table 6, it can be concluded that the best combination of A>D>B>C deproteinization is A>D>B>C. 1 B 3 C 3 D 1 , that is, when the enzyme-to-bottom ratio is 1%, the pH is 5.5, the enzymolysis time is 1.5h, and the temperature is 40 °C, the protein removal effect is the best.

Embodiment 3

[0064] Example 3 Protein removal by sevag method

[0065] Table 7 The factor level table of orthogonal experiment of sevag method

[0066]

[0067] Table 8 L 9 (3 4 ) Orthogonal experimental results

[0068]

[0069] From Table 8, the influence relationship is D>C>A>B, that is, the best deproteinization conditions: 4 times of deproteinization, 11min shaking time, sample solution: sevag reagent (v:v) = 2:1, chloroform : n-butanol (v:v)=5:1; the protein removal rate was 64.2%, and the polysaccharide loss rate was 34.3%.

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Abstract

The invention relates to a technical method for decoloring and deproteinizing corn stigma polysaccharide and belongs to the field of refining processing method for effective ingredients of plants, in particular to the method for decoloring and deproteinizing corn stigma polysaccharide during refining. The decoloring mainly refers to a decoloring method of the corn stigma polysaccharide by hydrogen peroxide superior to that by activated carbon. Compared with the decoloring method of the corn stigma, the method has the advantages that: the decoloring rate is 90.7 percent higher, and the polysaccharide loss rate is 18.80 percent lower. A deproteinizing method by sevag and enzyme is superior to that by pure sevag or pure enzyme. Compared with those of the deproteinizing method by pure sevag or pure enzyme, the deproteinizing rate of enzyme is 74.64 percent higher and the loss rate is 5.49 percent lower by the joint application of the deproteinizing method by sevag and the deproteinizing method by pure sevag or pure enzyme and the joint method reduces the dosage of enzyme, shortens enzymolysis time and reduces production cost. The method has the advantages of low cost of provided optimizing conditions, simple operation, good effect, less damage to the corn stigma polysaccharide and suitability for industrial production.

Description

technical field [0001] The invention belongs to the field of refining and processing methods of plant active ingredients, in particular to a method for decoloring and deproteinizing in the refining of corn silk polysaccharide. Background technique [0002] The "hair" of corn, that is, Stigma maydis, is the style and stigma of the grass plant Maize, and is a commonly used traditional Chinese medicine. Traditional Chinese medicine believes that corn silk has light taste, flat nature, diuretic, calming liver, and choleretic effects. Modern medical research believes that corn silk contains fatty oil, saponins, alkaloids, flavonoids, vitamin K3, ascorbic acid, organic acids, etc., which have diuretic, blood pressure lowering, blood sugar lowering, choleretic, hemostasis, antibacterial and immune enhancement, anticancer And many other effects. Scholars have studied the hypoglycemic effect of its water decoction. The content of corn silk polysaccharides in corn silk is up to 4%, ...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02
Inventor 周鸿立王亚红朱琳张艳张扬
Owner JILIN INST OF CHEM TECH
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