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Technological method for extracting caffeine and tea polyphenol mixture from tea step by step

A process method and technology for caffeine, which are applied in the field of step-by-step extraction of caffeine and tea polyphenol mixture, can solve the problems of difficult large-scale production, cumbersome process, low yield and the like, and achieve a low extraction temperature, simple process and high extraction rate. Effect

Inactive Publication Date: 2010-12-15
GUANGZHOU RZN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods all have disadvantages in varying degrees: for example, the product of the solvent extraction method may have residual organic solvents; the ion precipitation method not only has a low yield, but also uses metal salts to precipitate and separate tea polyphenols, and the metal salts in the product will be separated. There are different degrees of residue; the resin adsorption method is cumbersome and difficult to produce on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The steps and conditions of a process method for extracting a mixture of caffeine and tea polyphenols step by step from tea leaves are as follows:

[0026] Step 1: Extract caffeine

[0027] (1) After crushing 250 grams of dried tea leaves to 20 mesh, place them in 1 liter of supercritical CO 2 in the extraction kettle, and then turn on the CO 2 The high-pressure pump injects high-pressure CO into the extraction kettle at a flow rate of 3L / h 2 At the same time, open the entrainer pump and inject the entrainer that is water in the ratio of 1:0.1 according to the solid-liquid ratio into the extraction kettle;

[0028] (2) When the pressure in the extraction kettle rises to 20MPa, and the pressure is kept constant by adjusting the valve between the extraction kettle and the analysis kettle, the high-pressure CO containing the extract caffeine and the entrainer 2 The gas enters the desorption tank that has been kept at 40°C, and the CO in the desorption tank 2 After gas ...

Embodiment 2

[0034] The steps and conditions of a process method for extracting a mixture of caffeine and tea polyphenols step by step from tea leaves are as follows:

[0035] Step 1: Extract caffeine

[0036] (1) After crushing 250 grams of dried tea leaves to 30 mesh, place them in 1 liter of supercritical CO 2 in the extraction kettle, and then turn on the CO 2 The high-pressure pump injects high-pressure CO into the extraction kettle at a flow rate of 4L / h 2 gas, and simultaneously open the entrainer pump and inject the entrainer, i.e. 30% ethanol, into the extraction kettle by solid-liquid ratio of 1:0.5;

[0037] (2) When the pressure in the extraction kettle rises to 24MPa, and the pressure is kept constant by adjusting the valve between the extraction kettle and the analysis kettle, the high-pressure CO containing the extract caffeine and the entrainer 2 The gas enters the desorption tank that has been kept at 48°C, and the CO in the desorption tank 2 After separation of the ga...

Embodiment 3

[0043] The steps and conditions of a process method for extracting a mixture of caffeine and tea polyphenols step by step from tea leaves are as follows:

[0044] Step 1: Extract caffeine

[0045] (1) After crushing 250 grams of dried tea leaves to 40 mesh, place them in 1 liter of supercritical CO 2 in the extraction kettle, and then turn on the CO 2The high-pressure pump injects high-pressure CO into the extraction kettle at a flow rate of 5L / h 2 At the same time, open the entrainer pump and inject the entrainer that is water in the ratio of 1:0.6 according to the solid-liquid ratio into the extraction kettle;

[0046] (2) When the pressure in the extraction kettle rises to 28MPa, and the pressure is kept constant by adjusting the valve between the extraction kettle and the analysis kettle, the high-pressure CO containing the extract caffeine and the entrainer 2 The gas enters the desorption tank that has been kept at 55°C, and the CO in the desorption tank 2 After gas i...

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Abstract

The present invention discloses a method for extracting a mixture of tea polyphenol through soaking, wherein the mixture of tea polyphenol is extracted through the soaking method. The method comprises the following steps of (1) adding water or 30% of ethanol to the tea with the caffeine extracted according to a solid-to-liquid ratio of 1:5-8, soaking the mixture for the first time in the presence of normal temperature and microwave action; (2) adding water or 30% of ethanol to the filter residue after the first soaking and filtering operation according to a solid-to-liquid ratio of 1:3-5, soaking the mixture for 4 to 6 hours for the second time in the presence of a microwave with the heating temperature range thereof 65 to 80 DEG C; after 20-30 minutes since the completion of the microwave action, filtering, concentrating, and drying the mixture to obtain the mixture of tea polyphenol. The technical method for extracting the mixture of tea polyphenole from tea has no need of chemical solvent for separation, and can prepare pollution-free products as well as high-purity substances of catechin, tea polysaccharide and the like in the tea polyphenol mixture. The invention has the advantages of high extraction ratio, low cost, simple technology and easy massive preparation.

Description

technical field [0001] The invention relates to a method for extracting a mixture of caffeine and tea polyphenols, in particular to a process for step-by-step extraction of a mixture of caffeine and tea polyphenols from tea leaves. Background technique [0002] Tea polyphenols (Tea polphenols, TP) is a major natural compound in tea, and its content accounts for about 10%-35% of the total weight of dry matter in tea. Tea polyphenols are composed of catechins (flavanols), anthocyanins (anthocyanins and anthocyanins), anthocyanins (flavonoids and flavonols), fenac and concentrated fenac. The most valuable are catechins, accounting for about 60%-80% of the total amount of tea polyphenols. [0003] Studies have shown that tea polyphenols can scavenge free radicals in the human body and improve immunity. It has health care functions such as anti-oxidation, anti-aging, anti-radiation, and anti-cancer. It has been successfully used in medicine, health products, oil and food industr...

Claims

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Application Information

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IPC IPC(8): C07D473/12C07D311/62C08B37/00C07G99/00
CPCY02P20/54
Inventor 张海
Owner GUANGZHOU RZN BIOTECH CO LTD
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