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Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters

A simultaneous extraction and taurine technology, applied in chemical instruments and methods, cyanide reaction preparation, organic compound preparation, etc., can solve the problems of pollution, raw material toxicity and large environment, and achieve the effect of high degree of hydrolysis

Active Publication Date: 2010-12-15
QIANGJIANG YONGAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, taurine is mainly produced by chemical synthesis at home and abroad, but there are serious problems such as high toxicity of raw materials and environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting and preparing biological zinc, natural taurine and polypeptide from oysters, comprising the following steps:

[0025] a. Extraction of biological zinc: Weigh 1000g of oyster meat, put it into a container, add 3000g of pure water, and then crush it with an ultrasonic crusher with a power of 80w and a time of 20min.

[0026] b. Separation and concentration of biological zinc: Centrifuge the crushed oyster liquid to take the supernatant, concentrate the supernatant by rotary evaporation, and then freeze-dry at -30°C to obtain 11.19g of biological zinc powder, which contains zinc 63.02%, free amino acid 12.83%.

[0027] c. Prepare oyster slurry: add 3000 g of pure water to the centrifuged oyster meat, mash and homogenate.

[0028] d. Boiling to extract taurine: control the oyster homogenate to cook at 60° C. for 2 hours. Centrifuge the cooked oyster slurry to take the supernatant, then add 3000g of pure water, take the supernatant after cooking at ...

Embodiment 2

[0037] It is basically the same as Example 1, except that during the ultrasonic treatment process, the power of the ultrasonic wave is 70w, and the treatment time is 25min; the enzyme added in the first step of enzymolysis in the polypeptide preparation is papain, and the temperature is controlled at 55 °C, pH 6.0. Finally, 10.25g of biological zinc was obtained, containing 58.29% of zinc and 13.50% of free amino acids; 2.95g of taurine, with a purity of 97.5%; 65.70g of polypeptide products, including 38.57% of polypeptides and 10.54% of free amino acids.

Embodiment 3

[0039]It is basically the same as Example 1, except that 2000 g of pure water is added when extracting biological zinc, preparing slurry, and preparing polypeptide. Finally, 8.55g of biological zinc was obtained, which contained 60.40% of zinc and 11.20% of free amino acids; 1.53g of taurine, with a purity of 98.5%; 58.75g of polypeptide products, which contained 36.65% of polypeptides and 12.70% of free amino acids.

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PUM

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Abstract

The invention discloses a method for extracting and preparing taurine, biological zinc and polypeptide from oysters, comprising the following steps of: separating biological zinc and free amino acid in oyster meat through ultrasonic crushing; poaching the uniformly pulped oyster meat to release the taurine from cells, and then carrying out resin purification and concentrated crystallization to obtain high-purity taurine; and hydrolyzing the residual oyster meat in a bienzyme hydrolysis mode to obtain an oyster extractive rich in polypeptide. The extraction method not only greatly reserves natural active substances in the oysters, but also solves the problem of inapplicability for partial crowds because the oysters do not contain easy-sensitivity albumen and purine, and lays the foundationfor the application and the development of the oyster extractive in beverages or foods.

Description

technical field [0001] The invention relates to the extraction, separation and preparation of three active substances such as biological zinc, taurine and polypeptide, in particular to a method for synchronously extracting the three active substances from oysters. Background technique [0002] Oysters are warm-water bivalve molluscs. Their meat is delicious and nutritious. It is rich in glycogen, protein, amino acids, vitamins, biological zinc, trace elements and other ingredients, and has high nutritional value. [0003] Taurine is one of the most abundant amino acids in oysters. Taurine, also known as 2-aminoethanesulfonic acid, is a special amino acid with a non-protein structure and one of the important amino acids necessary for the human body. It generally exists in various tissues in animals in the form of free amino acids. Taurine is a protective agent against oxidative damage. It can scavenge oxygen free radicals, inhibit lipid peroxidation, maintain cell osmosis an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C309/14C07C303/44C07C229/00C07C227/40C12P21/06
Inventor 柯珂王一兵何碧娟庄军莲邱绍芳王俊
Owner QIANGJIANG YONGAN PHARMA
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