Loofah beer
A technology of loofah and beer, which is applied in the field of beer to achieve the effects of pure taste, plaque elimination and Japanese encephalitis prevention
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Embodiment 1
[0017] (1) Preparation of loofah juice: Wash 130 kg of loofah with water and break it to obtain loofah juice. Add 0.078 kg of loofah juice to the loofah juice with an activity of 9500 U of cellulase, and enzymolyze 40 Filtrate in 1 minute, sterilize the filtrate at 137°C for 3 seconds, then rapidly cool to 32°C to obtain loofah juice, cover with plastic wrap, and set aside.
[0018] The assay method of cellulase activity is common knowledge to those skilled in the art. In order to clearly explain the present invention, the assay method of cellulase activity is now described.
[0019] The activity of cellulase is 9500U, that is, at pH 4.5, 40°C, the amount of enzyme that decomposes filter paper to produce 1 microgram of glucose per minute is 1 unit of enzyme. Enzyme activity assay steps:
[0020] a: The enzyme preparation is prepared with 0.04M acetic acid-sodium acetate buffer solution with a pH of 4.5 to prepare a 0.20 mg / ml enzyme solution;
[0021] b: Take 0.5, 1.0, 1.5, ...
Embodiment 2
[0033] (1) Preparation of loofah juice: wash 130 kg of loofah with water and break it to obtain loofah juice. Add cellulase with an activity of 10500U accounting for 0.13 kg of loofah juice to the loofah juice, and enzymolyze 40 U under the condition of 30 ° C. Filtrate in 1 minute, sterilize the filtrate at 137°C for 5 seconds, then rapidly cool to 32°C to obtain loofah juice, cover with plastic wrap, and set aside.
[0034] The activity measurement of cellulase is the same as embodiment 1
[0035] (2) Preparation of wort: put 1,950 kg of barley malt, 650 kg of rice, 3.9 kg of hop oil, and 7,280 kg of water into the mash pot, rise from normal temperature to 45°C within 10 minutes, keep warm for 60 minutes, and then Raise the temperature from 45°C to 65°C within 15 minutes, keep it warm for 40 minutes, then raise the temperature from 65°C to 70°C within 15 minutes, keep it warm for 20 minutes, then raise the temperature from 70°C to 80°C within 15 minutes, keep it warm for 15 ...
Embodiment 3
[0039] (1) Preparation of loofah juice: wash 130 kg of loofah with water and break it to prepare loofah juice. Add cellulase with an activity of 10000U accounting for 0.10 kg of loofah juice to the loofah juice, and enzymolyze 40 U under the condition of 30 ° C. Filtrate in 1 minute, sterilize the filtrate at 137°C for 4 seconds, then rapidly cool to 32°C to obtain loofah juice, cover with plastic wrap, and set aside.
[0040] The activity measurement of cellulase is the same as embodiment 1
[0041] (2) Preparation of wort: put 1,690 kg of barley malt, 910 kg of rice, 1.3 kg of hop oil, and 7,950 kg of water into the mash pot, rise from normal temperature to 45°C within 10 minutes, keep warm for 50 minutes, and then Raise the temperature from 45°C to 65°C within 15 minutes, keep it warm for 30 minutes, then raise the temperature from 65°C to 70°C within 15 minutes, keep it warm for 30 minutes, then raise the temperature from 70°C to 80°C within 15 minutes, keep it warm for 10 m...
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