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Embedding immobilization method for microbial preparation

A microbial preparation and microbial technology, applied in the field of bioengineering, can solve the problems of inapplicability of macromolecular substrates, hinder the diffusion of substrates and oxygen, and achieve the effects of increasing strength and activity, increasing permeability, and increasing stability.

Inactive Publication Date: 2010-11-03
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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AI Technical Summary

Problems solved by technology

This method is simple to operate and has little effect on cell activity. It is the most widely studied immobilization method at present, but the embedding material will hinder the diffusion of substrate and oxygen to a certain extent, and it is not suitable for macromolecular substrates.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] a) Rinse the straw, cut it off, and dry it in a blast dryer. After the straw completely loses moisture, grind it into a powder in a blender, and then sterilize it.

[0025] b) Weigh 80g of polyvinyl alcohol into 1000mL of distilled water and dissolve completely at 80°C, then add 4g of sodium alginate, 8g of silicon dioxide, and 0.5g of calcium carbonate and mix well.

[0026] c) In 250mL of microbial solution (each milliliter containing microorganisms is 8×10 6 CFU) was added 5g of activated carbon and 5g of straw powder and mixed for 10 minutes for adsorption.

[0027] d) When the gel solution is cooled to 35°C, mix the above two solutions evenly, and then drop them into the boric acid solution containing 2% calcium chloride (pH=6.7) with a glue tip dropper, and immobilize at 10°C After 24 hours, make immobilized pellets and wash them with normal saline for 2-3 times after pelleting. The pellet size is about 10mm,

[0028] e) The successfully produced immobilized pe...

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PUM

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Abstract

The invention discloses an embedding immobilization method for a microbial preparation. 1 liter of embedding material comprises 70 to 90g of polyvinyl alcohol, 3 to 5g of sodium alginate, 7 to 9g of silicon dioxide and 0.4 to 0.6g of calcium carbonate; 200 to 250ml of microbial biomass is added into each liter of gel solution (each milliliter of the microbial biomass is 7*10<6> to 9*10<6>CFU); 4 to 6g of activated carbon and 4 to 6g of straw powder are added into microbe solution; and the immobilized grain diameter is about 10mm. The embedding immobilization method has the advantages that microbes well grow in immobilized beads; immobilization materials have no toxic or side effects on aquaculture animals; the immobilization method is simple to operate; the immobilization materials are economic and practical and easy to recycle and reuse; immobilized microbes are easy to preserve and transport and if microbes capable of decontaminating wastewater are immobilized by the method, the immobilized microbes have purification effects superior to those in a suspension state.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to an immobilization method for microorganisms. Background technique [0002] Since the immobilization technology appeared in the 1950s, after decades of development, it has formed a relatively complete theory and method. The immobilization technology makes the biocatalyst have completely different characteristics from its free state: for example, it is easy to separate from the product. The biocatalyst can be recovered or recycled, the stability of the biocatalyst is greatly improved, and the reaction process can be strictly controlled. These characteristics greatly reduce the application cost of expensive biocatalysts, so that they can be applied in large-scale industrial production. The immobilization technology of cells is an extension of immobilized enzyme technology and is one of the important contents in the field of modern bioengineering. . Immobilized cells have the characte...

Claims

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Application Information

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IPC IPC(8): C12N11/04
Inventor 何连生孟睿席北斗
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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