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Production method for extracting tryptophan from fermentation liquor by one-step refining

A production method and technology of tryptophan, which is applied in the field of biochemical engineering, can solve the problems of low extraction yield, environmental pollution, and easy deterioration of products, and achieve the effects of high extraction yield, reduced cost, and less membrane pollution

Active Publication Date: 2010-10-20
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the problems of easy deterioration of the product, low extraction yield and serious environmental pollution in the industrialization process of the production of L-tryptophan by the existing fermentation method, and provides a method for extracting tryptophan in the fermentation liquid by a one-step refining method. production method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The first step is to pretreat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO to the volume of the fermentation broth respectively 3 Afterwards, stir and heat to 60°C for 30 minutes to make a treatment solution;

[0037] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99.3%;

[0038] The...

Embodiment 2

[0047] The first step is to pretreat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO to the volume of the fermentation broth respectively 3 Afterwards, stir and heat to 60°C for 30 minutes to make a treatment solution;

[0048] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99.3%;

[0049] Th...

Embodiment 3

[0058] The first step is to pretreat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO to the volume of the fermentation broth respectively 3 Afterwards, stir and heat to 60°C for 30 minutes to make a treatment solution;

[0059] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99.3%;

[0060] Th...

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Abstract

The invention discloses a production method for extracting tryptophan from fermentation liquor by one-step refining, which comprises the pretreatment of the fermentation liquor. In the method, mycoprotein is filtered off by using a ceramic microfiltration membrane, is decolorized by using an ultrafiltration membrane and is desalted and concentrated by using a nanofiltration membrane; the nanofiltration membrane concentrated liquor is directly decolorized by using activated carbon, the decolorized liquor is subjected to isoelectric point crystallization at a low temperature, and a qualified competitive product is obtained by rinsing with ice water during centrifugal separation; the crystallization mother liquor returns to the nanofiltration membrane for circulation; protein feed with high additional value is prepared by mixing, drying and crushing the ultrafiltration strong liquor and mycoprotein; and nanofiltration strong brine can be used as a culture medium for later use or sold serving as a base fertilizer. The method has the advantages of avoiding adding an organic solvent and an inorganic solvent in the preparation process of a crude product and a fine product, saving the solvent recovery cost, solving the problems of sewage treatment and environmental pollution, along with high utilization rate of the mother liquor, high product quality, and more contribution to industrial production, wherein the total extraction yield of L-tryptophan is 83.5 percent in a 500-ton production line.

Description

Technical field: [0001] The invention relates to the field of biochemical engineering, a new process for one-time purification, separation and extraction of L-tryptophan from fermentation broth in industrial production, in particular to a production method for extracting tryptophan from fermentation broth by one-step purification. Background technique: [0002] L-tryptophan is one of the eight essential amino acids in human and animal life activities. It plays an important role in growth, development and metabolism. It is called the second essential amino acid and is widely used in medicine, food and feed. According to the calculation of the Food and Agriculture Organization of the United Nations in 2000, the annual demand for L-tryptophan in the world market is about 1.2 million tons or more. In order to promote the application of L-tryptophan, L-tryptophan, whose price has always been relatively high, hopes to have a cheap and efficient production and extraction method. T...

Claims

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Application Information

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IPC IPC(8): C07D209/20A23K1/06C05F5/00A23K10/38
CPCY02A40/20Y02P20/582Y02P60/87
Inventor 邓立君赵春光孟宗盘
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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