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Preparation method of crocetin, gardenia yellow pigment and saffron pigment

A technology of gardenia yellow pigment and crocus acid, which is applied in chemical instruments and methods, preparation of sugar derivatives, separation/purification of carboxylic acid compounds, etc. Large-scale production and other issues

Inactive Publication Date: 2010-10-20
HANGZHOU FAHE MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Crocetin acid mainly exists in the precious Chinese herbal medicine saffron. Since the output of saffron is very small, my country mainly relies on imports, and it is impossible to realize the large-scale production of crocetin.

Method used

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  • Preparation method of crocetin, gardenia yellow pigment and saffron pigment
  • Preparation method of crocetin, gardenia yellow pigment and saffron pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation of embodiment one crocetin

[0016] Grind the fruits of the genus Gardenia, add alkaline water (adjust to pH ≈ 10 with NaOH, etc.), heat and extract to obtain extract I, and extract I to pH ≈ 3 with HCl, etc., and then extract with ethyl acetate, ether, etc. to obtain Extract II. The extract II is concentrated to produce a precipitate, and the crude product of crocetin is obtained by filtration, and the crude product of crocetin is recrystallized with different concentrations of methanol, ethanol, tetrahydrofuran, alkaline water, etc. to obtain the pure product of crocetin, with a purity greater than 99% ( figure 1 ).

Embodiment 2

[0017] The preparation of embodiment two crocetin and gardenia yellow pigment

[0018] Grind the fruits of the genus Gardenia, add acid water (adjust pH ≈ 3 with HCl, etc.) and heat to extract to obtain Gardenia yellow pigment. The dregs are added to alkaline water (adjusted to pH ≈ 10 with NaOH, etc.), heated and extracted to obtain extract I, which is adjusted to pH ≈ 3 with HCl, etc., and then extracted with ethyl acetate, ether, etc. to obtain extract. The extract is concentrated to produce a precipitate, and the crude crocetin is obtained by filtration, and the crude crocetin is recrystallized with different concentrations of methanol, ethanol, tetrahydrofuran, alkaline water, etc. to obtain pure crocetin with a purity greater than 99% ( figure 1 ).

Embodiment 3

[0019] The preparation of embodiment three crocetin

[0020] Grind the fruits of the genus Gardenia, add alkaline alcohol (70-80% ethanol is adjusted to pH ≈ 10 with NaOH, etc.) and heat to extract to obtain an alcohol extract. The alcohol extract is recovered until the alcohol content is less than 10%, and the low-polar substances are washed away with ethyl acetate or ether. The aqueous solution was adjusted to pH ≈ 3 with HCl, and then extracted with ethyl acetate, ether, etc. to obtain an extract. The extract is concentrated to produce a precipitate, and the crude crocetin is obtained by filtration, and the crude crocetin is recrystallized with different concentrations of methanol, ethanol, tetrahydrofuran, alkaline water, etc. to obtain pure crocetin with a purity greater than 99% ( figure 1 ).

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Abstract

The invention provides a method for efficiently separating crocetin from fruits of rubiaceous plants and gardenia (stenophyllous gardenia, spoonleaf gardenia, gardenia hainanensis, gardenia sootepensis and gardenia) plants, saffron and saffron tissue cultures. The crocetin can be prepared by the method, and meanwhile, the gardenia yellow pigment and the saffron pigment can be prepared. The method comprises the following steps: extracting the pigment of gardenia fruits (the saffron and the saffron tissue cultures) through acid water or directly extracting raw materials through alkaline water, then carrying out water liquid acidification, then extracting ethyl acetate to obtain a crocetin crude product, and recrystallizing the crude product through different solvents to obtain a crocetin pure product. The crocetin, the gardenia yellow pigment and the saffron pigment provided by the invention can be applied in preparing cardiovascular and cerebrovascular disease medicaments, gynecological disease medicaments, tumor adjuvant therapy medicaments and foods. The method of the invention has reasonable design, the crocetin, the gardenia yellow pigment and the saffron pigment can be obtained in large scales and have high yield, and the method is quite suitable for industrialized production.

Description

technical field [0001] The invention relates to a preparation method of crocetin, gardenia yellow pigment and crocin pigment, which can be used in the development of medicine, cosmetics and food, in particular to the fruit of the Rubiaceae Gardenia plant and the A method for large-scale preparation of crocetin, gardenia yellow pigment and saffron pigment in safflower and saffron tissue culture fluid. Background technique [0002] In recent years, a large number of studies have shown that crocetin has the functions of promoting gallbladder, protecting liver, anti-tumor, enhancing memory, lowering blood pressure, lowering blood fat, anti-thrombotic, and immune regulation. It can also be used to treat hypertension, coronary heart disease, hyperlipidemia, menstrual Irregularity, amenorrhea, dysmenorrhea, postpartum lochia, warm toxin spots, bruises, depression, depression, palpitations and madness. Crocetin acid mainly exists in the precious Chinese herbal medicine saffron. Sin...

Claims

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Application Information

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IPC IPC(8): C07C57/13C07D311/30C07H13/06C07C51/42C07D311/40C07H1/08C07C51/43
Inventor 金涛
Owner HANGZHOU FAHE MEDICINE SCI & TECH
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