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Skin-whitening agent containing platycodin D

A technology of platycodon saponin and skin, applied in the field of skin whitening compositions, can solve the problems that the active ingredients have not been identified, and the same effect cannot be expected, and achieve excellent melanin production inhibition efficiency, excellent tyrosinase activity inhibition, and no toxicity and side effects

Active Publication Date: 2013-05-01
BIOSPECTRUM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most skin whitening compositions containing this natural herb extract are colored and have problems in incorporation, and the active ingredient has not been identified, so there is a problem that the same effect cannot be expected in the manufacture of products.

Method used

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  • Skin-whitening agent containing platycodin D
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  • Skin-whitening agent containing platycodin D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Determination of melanin production inhibitory effect by platycodon saponin-D

[0046] Using rat pigment cells (B16 melanoma cells), the melanin production inhibitory effect of Platycodon saponin-D (Sigma) was measured, and the measured effect was compared with the melanin production inhibitory effect of vitamin C, which is known to inhibit melanin production. Compare.

[0047] In this experiment, 1×10 per well on a 6-well plate 5 Rat melanoma (B16 F10) cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum) in 5% CO 2 and cultured at 37 °C until the cells adhere approximately 80% at the bottom of the wells. After culturing, the medium was removed and replaced with DMEM medium diluted with Platycodon saponin-D and vitamin C to an appropriate concentration, in 5% CO 2 , and cultured at 37°C for 3 days. The concentration range of Platycodon saponin-D was determined at 0.1 μM, 0.5 μM, 1 μM without cytotoxicity. Like Platycodon saponin-D...

Embodiment 2

[0051] Example 2: Determination of Inhibitory Effect of Intracellular Tyrosinase Activity Produced by Campanulaceae Saponin-D

[0052] Using rat pigment cells (B16 melanoma cells), the melanin production inhibitory effect by Platycodon saponin-D was measured, and the measured effect was compared with the inhibition of intracellular tyrosinase activity by vitamin C, which is known to inhibit melanin production. The effect is compared.

[0053] In this experiment, 1×10 per well on a 6-well plate 5 Inoculate mouse melanoma (murine melanoma) (B16F10) cells in DMEM medium containing 10% FBS (fetal bovine serum) in 5% CO 2 and cultured at 37 °C to allow the cells to adhere approximately 80% more to the bottom of the well. After culturing, the medium was removed and replaced with DMEM medium diluted with Platycodon saponin-D and vitamin C to an appropriate concentration, in 5% CO 2 , and cultured at 37°C for 3 days. The concentrations of platycodon saponin-D and vitamin C were 0....

Embodiment 3

[0057] Example 3: Evaluation of whitening effect at animal level

[0058] Using brown guinea pigs (Tortoiseshell guinea pigs; Brown guinea pigs), which are known to induce pigmentation by ultraviolet rays similarly to humans, the whitening effect by platycodon saponin-D was measured.

[0059] In order to induce hyperpigmentation produced by ultraviolet rays (UV) in the brown guinea pig, a 3×3 cm 2 shading aluminum foil with a square opening, and then irradiate ultraviolet light with SE lamp (wavelength 290-320nm, Toshiba) (total irradiation energy = 1350mJ / m 2 ). After the ultraviolet irradiation, the aluminum foil was peeled off, and the sample ( platycodon saponin-D and vitamin C) was coated by the following method. Pigmentation appears after 2 or 3 days of UV exposure, and reaches its peak after about 2 weeks. From this point on, each sample was coated.

[0060] The coating was collected once or twice a day for 50 consecutive days. The sample was dissolved and diluted ...

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Abstract

The present invention relates to cosmetic and pharmaceutical compositions which include platycodin D as an active ingredient. The platycodin D of the present invention can be used safely and without side effects on the skin, and also has outstanding effects in suppressing the production of melanin and impeding pigment deposition, and thus a composition which contains the present invention as an active ingredient can be used very effectively to improve skin patches and freckles and for skin whitening.

Description

technical field [0001] The present invention relates to a composition for skin whitening; more specifically, to a cosmetic and pharmaceutical composition for skin whitening which is excellent in product stability and contains platycodin-D, which has an effect on the skin. It is safe to use without side effects, and has excellent pigmentation inhibition effect by suppressing melanin production. Background technique [0002] Human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, it is also affected by environmental or physiological conditions such as solar ultraviolet rays, fatigue, and stress responses. Melanin produced by human skin melanocytes is a phenolic macromolecular substance in a complex form of black pigment and protein, and plays an important role in blocking skin damage caused by ultraviolet rays. It is reported that in the biosynthesis of melanin, the role of tyrosinase present in melanocytes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7034A61K8/60A61P17/00A61Q19/02
CPCA61K8/63A61K31/7034A61Q19/02A61P17/00A61P17/16
Inventor 朴德勋郑恩先李钟星
Owner BIOSPECTRUM
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