Method for identifying quality of ova of Acanthogobius ommaturus
A technology for spiny goby and spiny shrimp, applied in biochemical equipment and methods, microbe determination/inspection, color/spectral characteristic measurement, etc., can solve the problem of lack of identification of aquatic biological eggs, cumbersome process, and long test period and other issues, to achieve the effect of reducing human and external environmental factors, saving time and cost, and low price
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[0006] The present invention collects several tails of mature parents in the breeding season of the goby, takes 5 mature eggs of each batch, prepares 10% tissue homogenate after ultrasonic centrifugation, and uses a kit (Nanjing Jiancheng Biological Engineering Co., Ltd. Institute) measured the activities of total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) in different batches of mature eggs with a spectrophotometer, and each sample was repeated. Three times, the results were averaged, and statistical software was used to analyze and process the results. If the activity of total ATPase in the egg is higher than 36.31±2.50U / mgprot, the activity of CK is higher than 0.12±0.008U / mgprot, the activity of SDH is higher than 17.875±0.559U / mgprot, and the activity of LDH is higher than 191.876±10.672U / gprot, it is judged that the egg quality is better; if the ATPase activity in the egg is between 7.006±0.414U / mgprot and 36.31±2.50U / mgp...
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