Method for identifying quality of ova of Acanthogobius ommaturus

Abstract

The invention provides a method for identifying the quality of the ova of Acanthogobius ommaturus, which relates to an aquaculture technology and needs to provide a method for identifying the quality of the ova of Acanthogobius ommaturus. In the invention, mature ova are collected to prepare tissue homogenate in the mating season of the Acanthogobius ommaturus. The method is characterized in that a kit is used for measuring the activities of total ATP enzymes, creatine kinase, succinate dehydrogenase and lactate dehydrogenase in the ova to judge the quality of the ova. The invention is used for identifying the quality of the ova of the Acanthogobius ommaturus.

Description

technical field [0001] The invention belongs to the aquaculture technology, and relates to a method for identifying the egg quality of the spiny goby. Background technique [0002] In aquaculture production, the quality of the eggs of aquatic organisms directly affects the energy level, which in turn affects the emergence rate and survival rate. The quality of the eggs is closely related to the production efficiency. Therefore, it has important theoretical significance and application value to correctly evaluate the egg quality of aquatic organisms. At this stage, reports on the quality evaluation of aquatic organism eggs are relatively rare. Generally, tissue sections are stained for microscopic observation. It can not directly guide the production; another method is to boil the eggs of aquatic organisms in boiling water, cut the eggs from the middle with a blade, and observe that the inner core of the eggs is extremely biased toward the animal. According to the situation...

AI Technical Summary

Problems solved by technology

At this stage, reports on the quality evaluation of aquatic organism eggs are relatively rare. Generally, tissue sections are stained for microscopic observation. It can not directly guide the production; another method is to boil the eggs of aquatic organisms in boiling water, cut the eggs from the middle with a blade, and observe that the inner core of the eggs is extremely biased toward the animal. According to the situation of migration, calculate the polarization index, and infer the developmental maturity of the egg based on this, but this method can only judge the maturity of the egg artificially, and cannot distinguish the quality of the egg development

Another method is to directly fertilize eggs with normal sperm, and calculate the fertilization rate and membrane production rate, but this method has a long test period and is easily interfered by external environmental factors, which may affect the final statistical results

Therefore, so far, there is still a lack of an effective method to identify the quality of aquatic organism eggs