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Method for identifying quality of ova of Acanthogobius ommaturus

A technology for spiny goby and spiny shrimp, applied in biochemical equipment and methods, microbe determination/inspection, color/spectral characteristic measurement, etc., can solve the problem of lack of identification of aquatic biological eggs, cumbersome process, and long test period and other issues, to achieve the effect of reducing human and external environmental factors, saving time and cost, and low price

Inactive Publication Date: 2010-09-29
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

At this stage, reports on the quality evaluation of aquatic organism eggs are relatively rare. Generally, tissue sections are stained for microscopic observation. It can not directly guide the production; another method is to boil the eggs of aquatic organisms in boiling water, cut the eggs from the middle with a blade, and observe that the inner core of the eggs is extremely biased toward the animal. According to the situation of migration, calculate the polarization index, and infer the developmental maturity of the egg based on this, but this method can only judge the maturity of the egg artificially, and cannot distinguish the quality of the egg development
Another method is to directly fertilize eggs with normal sperm, and calculate the fertilization rate and membrane production rate, but this method has a long test period and is easily interfered by external environmental factors, which may affect the final statistical results
Therefore, so far, there is still a lack of an effective method to identify the quality of aquatic organism eggs

Method used

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Experimental program
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Embodiment Construction

[0006] The present invention collects several tails of mature parents in the breeding season of the goby, takes 5 mature eggs of each batch, prepares 10% tissue homogenate after ultrasonic centrifugation, and uses a kit (Nanjing Jiancheng Biological Engineering Co., Ltd. Institute) measured the activities of total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) in different batches of mature eggs with a spectrophotometer, and each sample was repeated. Three times, the results were averaged, and statistical software was used to analyze and process the results. If the activity of total ATPase in the egg is higher than 36.31±2.50U / mgprot, the activity of CK is higher than 0.12±0.008U / mgprot, the activity of SDH is higher than 17.875±0.559U / mgprot, and the activity of LDH is higher than 191.876±10.672U / gprot, it is judged that the egg quality is better; if the ATPase activity in the egg is between 7.006±0.414U / mgprot and 36.31±2.50U / mgp...

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Abstract

The invention provides a method for identifying the quality of the ova of Acanthogobius ommaturus, which relates to an aquaculture technology and needs to provide a method for identifying the quality of the ova of Acanthogobius ommaturus. In the invention, mature ova are collected to prepare tissue homogenate in the mating season of the Acanthogobius ommaturus. The method is characterized in that a kit is used for measuring the activities of total ATP enzymes, creatine kinase, succinate dehydrogenase and lactate dehydrogenase in the ova to judge the quality of the ova. The invention is used for identifying the quality of the ova of the Acanthogobius ommaturus.

Description

technical field [0001] The invention belongs to the aquaculture technology, and relates to a method for identifying the egg quality of the spiny goby. Background technique [0002] In aquaculture production, the quality of the eggs of aquatic organisms directly affects the energy level, which in turn affects the emergence rate and survival rate. The quality of the eggs is closely related to the production efficiency. Therefore, it has important theoretical significance and application value to correctly evaluate the egg quality of aquatic organisms. At this stage, reports on the quality evaluation of aquatic organism eggs are relatively rare. Generally, tissue sections are stained for microscopic observation. It can not directly guide the production; another method is to boil the eggs of aquatic organisms in boiling water, cut the eggs from the middle with a blade, and observe that the inner core of the eggs is extremely biased toward the animal. According to the situation...

Claims

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Application Information

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IPC IPC(8): C12Q1/42C12Q1/50C12Q1/32G01N21/31
Inventor 黄晓荣章龙珍庄平江琪姚志峰刘鉴毅张涛冯广朋赵峰闫文罡
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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