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Method for identifying vitality of sperm of Nibea albiflora

A technology of sperm motility and yellow croaker, which is applied in the fields of biochemical equipment and methods, microbiological determination/inspection, color/spectral characteristic measurement, etc. It can solve the problem of large impact, inability to objectively reflect the status of sperm activity, expensive equipment, etc. problem, to achieve the effect of short time required, reduction of subjective factors, and large number of samples

Inactive Publication Date: 2010-09-22
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

When evaluating fish sperm motility, there are mainly the following methods at present: 1. Use ordinary microscopes to observe and record the activity status and movement time of sperm. This method is greatly affected by human factors and cannot objectively reflect the activity status of sperm; 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will be longer with the length of early development time of different fish Big changes; 3. Recently, a computer-aided analysis system (CASA) has been developed to analyze the movement rate and distance of sperm. This method can objectively reflect the movement of sperm, but the equipment is relatively expensive.

Method used

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Experimental program
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Embodiment Construction

[0006] During the breeding season of yellow croaker, the sperm of croaker was collected in batches, and the sperm of 5 fishes were taken in each batch, and the kits (produced by Nanjing Jiancheng Bioengineering Institute) were used to measure the sperm of different batches under the ultraviolet spectrophotometer. The activities of total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH) and acid phosphatase (ACP) in spermatozoa were repeated three times for each sample, and the results were averaged , using statistical software to analyze and process the results, if the activity of ATPase in sperm is higher than 6.34±1.41U / ml, the activity of CK is higher than 7.86±0.36U / ml, and the activity of SDH is higher than 5.67±1.15U / ml, The activity of LDH is higher than 2120.53±101.26U / L, and the activity of ACP is higher than 14.74±1.70U / 100ml, which means that the sperm motility is better; if the activity of ATPase in sperm is between 0.32±0.07U / ...

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Abstract

The invention provides a method for identifying the vitality of the sperm of Nibea albiflora, relates to the technical field of aquaculture. The method uses a kit and an ultraviolet spectrophotometer, and is characterized in that: in the breeding season of the Nibea albiflora, a certain amount of sperm is collected by batch; and the activities of total ATPase, creatinekinase, succinodehydrogenase, lactic dehydrogenase and acid phosphatase in different batches of sperm are tested respectively by the kit to differentiate sperm with high vitality, sperm with medium vitality and sperm with low vitality. The method is used for identifying the vitality of the sperm of the Nibea albiflora.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for identifying the sperm activity of yellow croaker. Background technique [0002] In production practice, the vitality of fish sperm is one of the important indicators reflecting the fertilization rate. Therefore, objective and accurate evaluation of the vitality of fish sperm has very important theoretical significance and application value. When evaluating fish sperm motility, there are mainly the following methods at present: 1. Use ordinary microscopes to observe and record the activity status and movement time of sperm. This method is greatly affected by human factors and cannot objectively reflect the activity status of sperm; 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/50C12Q1/32C12Q1/42C12Q1/25G01N21/31
Inventor 黄晓荣章龙珍庄平江琪姚志峰刘鉴毅张涛冯广朋赵峰闫文罡
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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