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New use of cell line LNCaP and cell line C4-2

A cell line and cell technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve problems such as different genetic backgrounds, inactivation of AR signaling pathways, and non-expression of AR proteins

Inactive Publication Date: 2010-09-15
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Abstract
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Problems solved by technology

But there is a problem that the genetic background of LNCaP is different from that of PC3 or DU145 cells
However, AR protein is not expressed in PC3 or DU145 cells, and the AR signaling pathway is inactivated
Therefore, the differentially expressed genes among them may not accurately represent the determinant genes associated with the progression of radiosensitivity in prostate cancer cell lines.

Method used

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  • New use of cell line LNCaP and cell line C4-2
  • New use of cell line LNCaP and cell line C4-2

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1, LNCaP cell and C4-2 cell resistance detection to ionizing radiation

[0034] 1. C4-2 cells have stronger anti-ionizing radiation growth ability

[0035] In order to understand the response of the LNCaP / C4-2 cell model to ionizing radiation, the MTT experiment analyzed the growth of the cells cultured at different times after 0, 2.5, 5 and 10 Gy radiation.

[0036] The LNCaP cells and C4-2 cells were subjected to ionizing radiation at doses of 0, 2.5, 5 and 10 Gy, respectively, and placed at 37°C, 5% CO 2 Culture in the incubator for 0, 6, 12, 24, 48, 72 hours. Add 20 μl of MTT solution (MTT solution is obtained by dissolving MTT in PBS, the concentration of MTT is 5mg / ml) to the above-mentioned cultured cells at different time points, in 5% CO 2 After culturing in the incubator for 4 hours, dissolve it with 150 μl DMSO, take 150 μl of the cell suspension and read the OD at 490 nm wavelength in an enzyme-linked analyzer 490 Value, with time as the horizo...

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Abstract

The invention discloses new use of a cell line LNCaP and a cell line C4-2. The new use is the use of the cell line LNCaP and the cell line C4-2 in the preparation of a prostate cancer cell model which is sensitive to radiation and generates resistance to the radiation. The new use of the cell line LNCaP and the cell line C4-2 demonstrates a new characteristic, namely the resistance of theC4-2 cells to ionizing radiation, of the LNCaP / C4-2 cell model first, and the LNCaP / C4-2 may be the cell model which represents the prostate cancer cells developing from IR sensitivity into IR resistance. The new use of the cell line LNCaP and the cell line C4-2 can be used for selecting an ionizing radiation induced gene which determines that the prostate cancer cells developing from the radiation sensitivity into the radiation resistance so as to select relative genes which take part in and determine the progression of the prostatic cancer, and thus, the first stone for discovering more effective clinic therapeutic target with hormone resistance and radiation resistance is laid.

Description

technical field [0001] The present invention relates to new uses of the cell line LNCaP and the cell line C4-2. Background technique [0002] In the treatment of prostate cancer (Prostate Cancer, prostate cancer), radiotherapy has always played an important role. Radiation therapy for early prostate cancer can achieve the goal of radical cure, and the curative effect is similar to radical surgery for prostate cancer. For locally advanced prostate cancer (especially those with distant metastasis), although radiotherapy can alleviate symptoms and improve the quality of life, it shows radiotherapy resistance. 30% of prostate cancer patients cured by radiotherapy will relapse at the original irradiation site. The sensitivity or resistance of tumor patients to ionizing radiation (IR) varies greatly among individuals, and individual radiation sensitivity or resistance cannot be reliably predicted before treatment, resulting in uncertain radiotherapy response. The mechanism of wh...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12N13/00
Inventor 周建光施庆国
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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