Humanized neutralizing antibody (RVFab8) against rabies virus glycoprotein

A rabies virus, glycoprotein technology, applied in the direction of antiviral immunoglobulin, antiviral agent, antibody, etc., can solve the problems of infection, limited application, and limitation of mass production

Active Publication Date: 2012-07-04
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most McAbs are of mouse origin, and the heterogeneous reaction of mouse McAbs greatly limits the application of McAbs as therapeutic agents in humans.
As an antibody component, immunoglobulin (Vaccinia immune globulin, VIG) mainly comes from the immune serum of donors (convalescent patients), and it takes a lot of manpower and financial resources to obtain positive serum and pass the safety test, which makes its mass production difficult. Restricted, and because it comes from serum, it is prone to infection of blood-borne diseases

Method used

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  • Humanized neutralizing antibody (RVFab8) against rabies virus glycoprotein
  • Humanized neutralizing antibody (RVFab8) against rabies virus glycoprotein
  • Humanized neutralizing antibody (RVFab8) against rabies virus glycoprotein

Examples

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Embodiment Construction

[0026] The specific embodiments of the present invention will be described in further detail below in conjunction with the drawings and embodiments. The following examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0027] Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0028] Materials and Method

[0029] 1. Viruses, cells and vectors: rabies virus aG strain, CTN strain, CVS strain and ERA strain are provided by the Chinese Center for Disease Control and Prevention of Viral Disease Control. The human immunization strain PM strain is an imported human vaccine strain, and the cell used for the rabies virus neutralization experiment in vitro is BHK-21 (ATCC). The strain is XLI-Blue (Stratagene, USA), and the vector is pComb3 (40kb), provided by the Scripps Research Institute, USA. The baculovirus expression vector is pAC-L-Fc (German PROGE...

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Abstract

The invention discloses a humanized neutralizing antibody (RVFab8) against rabies virus glycoprotein, which is obtained through screening by utilizing phage display technology. The antibody specifically identifies the granule antigen of the rabies virus, is against the rabies virus glycoprotein G, has obvious immunofluorescence reaction and enzyme linked immunosorbent assay with the rabies virus and has the neutralizing activity function against rabies virus infection. The antibody can be prepared into the specific antibody drugs for preventing and treating rabies, thereby being clinically used for preventing and treating rabies caused by the rabies virus.

Description

Technical field [0001] The present invention relates to genetic engineering antibody technology, in particular to a human anti-rabies virus glycoprotein neutralizing antibody; the present invention also relates to the application of the antibody in the preparation of drugs for preventing or treating rabies. Background technique [0002] Rabies is a worldwide zoonotic disease caused by the rabies virus, once it becomes ill, 100% will die. Rabies is currently reported in 87 countries in the world, and more than 50,000 people die from rabies every year (Knobel DL, et al. 2005). Prevention of rabies after exposure is the main measure to prevent and treat rabies. For severely exposed people, the World Health Organization (World Health Organization, WHO) recommends the use of rabies vaccine injection combined with rabies immune globulin (RIG). The two types of RIGs currently in use are human rabies immune globulin (HRIG) and equine rabies immune globulin (ERIG). Because ERIG has ser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C12N15/13C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10G01N33/569A61K39/42A61P31/14
CPCC07K2317/21C07K16/10A61P31/14
Inventor 梁米芳孙丽娜陈哲李川李德新
Owner NCPC NEW DRUG RES & DEV
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