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Method for fast identifying frankliniella occidentalis

A Western flower thrips and a flower thrips technology are applied in the field of rapid identification of Western flower thrips to achieve the effects of good specificity and protection of the ecological environment

Inactive Publication Date: 2010-08-18
CHECKOUT & QUARANTINE TECH CENT YUNNAN ENTRY &EXIT CHECKOUT & QUARANTINE BUR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for fast identifying frankliniella occidentalis
  • Method for fast identifying frankliniella occidentalis
  • Method for fast identifying frankliniella occidentalis

Examples

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Embodiment 1

[0020] ——Application of western flower thrips specific primers F.OL1 / F.OR for PCR identification

[0021] Total DNA was extracted from six species of thrips: Western flower thrips, flower thrips, yellow-breasted thrips, eight-section yellow thrips, tobacco thrips, and large thrips. / F.OR for conventional PCR amplification. The total volume of the PCR reaction is 50 μL, including: 10×PCR Buffer 5 μL, 25 mMol / L MgCl2 4 μL, 2.5 mMol / L dNTP 2 μL, Taq polymerase 0.3 μL (Takara, 5U / μL), 20 pmol F.OL1 / F.OR each 2 μL, template DNA 2 μL, and then add sterile water to stop the final volume of 50 μL. The reaction conditions are: 95°C for 5Min; 94°C for 50s, 53°C for 40s, 72°C for 50s, a total of 35 cycles; and finally 72°C for 6Min.

[0022] The PCR amplification products were detected by 1.5% agarose gel electrophoresis, and the detection results were as follows: figure 1 , after PCR amplification of the total DNA of Thrips occidentalis, the target band consistent with the expected f...

Embodiment 2

[0024] ——Application of western flower thrips specific primers F.OL2 / F.OR for PCR identification

[0025] Total DNA was extracted from six species of thrips: Western flower thrips, flower thrips, yellow-breasted thrips, eight-section yellow thrips, tobacco thrips, and large thrips. / F.OR for conventional PCR amplification. The total volume of the PCR reaction is 50 μL, including: 10×PCR Buffer 5 μL, 25 mMol / L MgCl2 4 μL, 2.5 mMol / L dNTP 2 μL, Taq polymerase 0.3 μL (Takara, 5U / μL), 20 pmol F.OL2 / F.OR each 2 μL, template DNA 2 μL, and then add sterile water to stop the final volume of 50 μL. The reaction conditions are: 95°C for 5Min; 94°C for 50s, 53°C for 40s, 72°C for 50s, a total of 35 cycles; and finally 72°C for 6Min.

[0026] The PCR amplification products were detected by 1.5% agarose gel electrophoresis, and the detection results were as follows: figure 1 , after PCR amplification of the total DNA of Thrips occidentalis, the target band consistent with the expected f...

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Abstract

The invention discloses a method for fast identifying frankliniella occidentalis. The method is based on the identification results of forms of six kinds of adult thrips such as frankliniella occidentalis and the like for respectively carrying out PCR amplification by using two pairs of frankliniella occidentalis specific primers F.OL1 / F.OR and F.OL2 / F.OR to establish the method for fast identifying the frankliniella occidentalis. The invention has the advantages of high speed, accuracy and good specificity, provides an effective identification measure for the quarantine pest of the frankliniella occidentalis, and has important meaning for protecting the ecological environment.

Description

technical field [0001] The invention belongs to the technical field of insect identification, more specifically, the invention relates to a rapid identification method of thrips occidentalis (including its adults, nymphs and pupae). Background technique [0002] Western flower thrips Frankliniella occidentalis is a pest with extremely heterogeneous feeding habits. Its recorded hosts include 244 species of plants in 66 families. It is also the vector insect of various plant viruses such as tomato spotted wilt virus (TSWV). countries listed as quarantine pests. Western flower thrips is native to North America, and then widely spread in Europe, Asia (Japan, South Korea, etc.), Africa (Kenya, South Africa, etc.), Central and South America (Costa Rica, Colombia, etc.) and Oceania (Australia, New Zealand) and other places. In 2003, Thrips occidentalis was found on hot peppers in greenhouses in Beijing in my country, and later reports of this insect were also found in some parts o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 周力兵丁元明刘忠善寸东义王辉周剑
Owner CHECKOUT & QUARANTINE TECH CENT YUNNAN ENTRY &EXIT CHECKOUT & QUARANTINE BUR
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