Method for establishing and detecting amniotic fluid stem cell line of pig

Abstract

The invention provides a method for establishing an amniotic fluid stem cell line of a pig, which comprises the following steps: (1) carrying out primary separation culture on the amniotic fluid stem cell; (2) mechanically separating and purifying the primary amniotic fluid stem cell subjected to the separation culture; and (3) detecting and culturing the separated and purified amniotic fluid stem cell and establishing the amniotic fluid stem cell line of the pig. The invention provides a method of a quick, effective, safe and separated amniotic fluid stem cell, can provide a seed cell sourcefor researching tissue engineering and is expected to be used for research, production and application of the seed cell.

Description

Establishment and detection method of a porcine amniotic fluid stem cell line technical field The invention relates to a method for establishing a cell line, in particular to a method for establishing a porcine amniotic fluid stem cell line. Background technique As research on embryonic stem cells is bound and limited by ethical and moral issues, many researchers are trying to find new sources of stem cells. In 2003, Prusa et al. found OCT4-positive cells in amniotic fluid, indicating that pluripotent stem cells may exist in amniotic fluid. In 2007, Anthony et al. reported in the journal Nature Biotechnology that they had found a new source of stem cells that was easy to obtain and had a strong ability to proliferate. By screening and isolating CD117-positive cells in amniotic fluid in the second trimester, the researchers obtained a small number of cells with embryonic stem cell characteristics. They named these cells amniotic fluid stem cells (AmnioticFluidStemCell, AF...

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Problems solved by technology

Domestic research on amniotic fluid stem cells has just started, and there are still a lot of gaps. There are only sporadic reports on the isolation and culture of human amniotic fluid cells, research progress, etc.

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