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Fluorescent microscopic imaging method and system thereof

A microscopic imaging and fluorescence technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of low efficiency of spectral measurement, achieve the effect of reducing crosstalk, improving spatial resolution and image contrast

Inactive Publication Date: 2010-08-04
JIANGSU TIANNING PHOTON TECH
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a fluorescence microscopic imaging method and system, aiming to solve the problem of low spectral measurement efficiency of existing fluorescence microscopic imaging methods and systems

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  • Fluorescent microscopic imaging method and system thereof
  • Fluorescent microscopic imaging method and system thereof
  • Fluorescent microscopic imaging method and system thereof

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Embodiment Construction

[0013] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0014] The difference between the embodiment of the present invention and the prior art is that the excitation light is formed into an excitation light array point through a beam splitter, and the excitation light array point divides the imaging field of view of the sample into multiple sub-regions, and a multi-line parallel excitation is formed by using a line scanning method. For multiphoton fluorescence, dispersive elements are used to distinguish fluorescence according to different wavelengths, and the fluorescence spectrum information is recorded in parallel by the detector, so as to realize fa...

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Abstract

The invention is applied to the fluorescent microscopic imaging, and provides a fluorescent microscopic imaging method and a system thereof. The invention is characterized in that excitation light array points are formed from an excitation light by an optical splitter, an imaging view field of the sample is divided into a plurality of sub-regions by the excitation light array points, multi-line parallel excitation multiphoton fluorescences are formed in a line scanning manner, the fluorescences are separated from each other according to difference wavelengths by a dispersion element, the fluorescent spectrum information is parallel recorded by a detector, and a multiphoton fluorescent microscopic rapid imaging of high spectra resolution on the sample is realized in the full spectrum range.

Description

technical field [0001] The invention belongs to fluorescence microscopic imaging technology, in particular to a fluorescent microscopic imaging method and system. Background technique [0002] Fluorescence spectrometers have been widely used in biomedicine and substance interaction research. Each molecule or atom has its own spectral characteristics. Under the excitation of ultraviolet light, visible light or near-infrared light, it will produce visible fluorescence. Therefore, fluorescence spectroscopy can be used for material composition and structure identification, such as amino acids, proteins, lipids and biopolymers. Among them, near-infrared light (700-1000nm) can significantly improve the fluorescence imaging depth and spatial resolution of the sample, reduce damage to living samples, provide a higher image signal-to-noise ratio, and one wavelength can simultaneously excite molecules with different absorption spectra. Various fluorophores. Therefore, two-photon ex...

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Application Information

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IPC IPC(8): G01N21/64
Inventor 邵永红屈军乐牛憨笨
Owner JIANGSU TIANNING PHOTON TECH
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