Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof

A technology of recombinant baculovirus and PCV-2, applied in application, gene therapy, antiviral agent, etc., can solve the problem of no vaccine and achieve the effect of good genetic stability

Inactive Publication Date: 2012-07-18
NORTHWEST A & F UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no vaccine for the control of PCV-2

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof
  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof
  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and implement it, but the examples given are not intended to limit the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen genes and a preparation method and application thereof, in particular relates to a recombinant baculovirus comprising a sequence shown in SEQ ID NO.1 and displaying structural proteins ORF2 of the PCV-2 on the surface. The preparation method comprises steps of utilizing the recombinant baculovirus to inoculate insect cells, culturing for 48-72h, collecting infection cells, carrying out freeze thawing at 40DEG C below zero / 37DEG C, centrifuging, taking supernate to test virus PFU and adjusting the titer of the virus to 109PFU / ml to obtain a safe and effective gene engineered subunit vaccine for preventing PCV-2.

Description

technical field [0001] The invention relates to a recombinant baculovirus expressing PCV-2 immunogen gene and its preparation and application. Background technique [0002] Porcine circovirus (PCV) belongs to the genus Circovirus of the family Circoviridae and is a representative species of the genus Circovirus. DNA virus, the average particle diameter of which is 17nm, is the smallest animal virus found in veterinary medicine. [0003] PCV can be divided into two serotypes, PCV-1 and PCV-2. PCV-1 is non-pathogenic to pigs and is a non-pathogenic PCV. PCV-2 is pathogenic to pigs and can cause PMWS (multisystemic wasting syndrome in weaned piglets), also known as PMWS-related PCV. The disease broke out in Canada for the first time in 1991, and there were reports of the disease in many countries and regions in the world. At present, the disease exists in many pig farms in my country, causing serious economic losses to the pig industry in my country. [0004] Hamel et al. fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/34C12N15/866A61K48/00A61K39/12A61P31/20
Inventor 许信刚童德文张琪
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com