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Method for preparing spreading hedyotis herb polysaccharide

A technology of Hedyotis diffusa and polysaccharide is applied in the field of preparation of Hedyotis diffusa polysaccharides, which can solve the problems of affecting product quality, adding large amount, breaking polysaccharide chains, etc., so as to achieve shortened heating time, high product content, and reduced usage amount. Effect

Inactive Publication Date: 2010-08-04
SUZHOU HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, in the process of preparing Hedyotis diffusa polysaccharides, there are often problems such as heating for a long time and adding a large amount of organic solvents, which is not conducive to energy saving and emission reduction, and may cause polysaccharide chain breaks and affect product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take Hedyotis diffusa, add distilled water 3 times its weight, and extract in a microwave extraction device with an extraction power of 300W, extract once, 3 minutes each time, combine the extracts, filter, and absorb through AB-8 macroporous adsorption resin , eluted with distilled water, collected 3 times the eluate of the column volume, ultrafiltered and concentrated through a polysulfone ultrafiltration membrane with a molecular cut-off of 5000 Daltons, added ethanol to the concentrated solution to make the alcohol content of the solution reach 70%, statically Set aside for 24 hours, filter, take the filter residue, wash and dry to obtain 112.4 g of Hedyotis diffusa polysaccharide, the content measured by the method of Test 1 is 87.4%.

Embodiment 2

[0022] Take Hedyotis diffusa, add 6 times its weight in distilled water, extract in a microwave extraction device, extract power 600W, extract 3 times, 10 minutes each time, combine extracts, filter, absorb through macroporous adsorption resin, wash with distilled water Remove, collect 8 times the eluate of the column volume, pass through the polysulfone ultrafiltration membrane ultrafiltration and concentration with a molecular cut-off of 50,000 Daltons, add ethanol to the concentrated solution to make the alcohol content of the solution reach 90%, and let it stand for 24 hours , filtered, and the filter residue was taken, washed and dried to obtain 100.7 g of Hedyotis diffusa polysaccharide, the content of which was 91.7% as measured by the method of Test 1.

Embodiment 3

[0024] Take 10Kg of Hedyotis diffusa, add 5 times of its weight in distilled water, extract in a microwave extraction device, extract power 450W, extract 2 times, 5 minutes each time, combine the extracts, filter, and pass through AB-8 macroporous adsorption resin Adsorption, elution with distilled water, collecting eluate with 5 times the volume of the column, ultrafiltration and concentration through a polysulfone ultrafiltration membrane with a molecular cut-off of 30,000 Daltons, adding ethanol to the concentrated solution to make the alcohol content reach 80%, static Set aside for 24 hours, filter, take the filter residue, wash and dry to obtain 146.8 g of Hedyotis diffusa polysaccharide, the content measured by the method of Test 1 is 89.3%.

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PUM

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Abstract

The invention relates to a method for preparing spreading hedyotis herb polysaccharide, which has the advantages of simple and convenient operation, low pollution and small energy consumption. The method comprises the following process steps: taking spreading hedyotis herb; adding distilled water into the spreading hedyotis herb; putting the mixture into a microwave extraction apparatus for extraction; merging extract liquid; filtering the combined extract liquid; adsorbing the filtrate by using macroporous adsorption resin; eluting the product by using the distilled water; performing ultrafiltration and concentration by using a polysulfone ultrafiltration membrane with the molecule interception between 5,000 and 50,000 Dalton; adding ethanol into concentrated solution to ensure that the alcohol content of the solution is up to 70 to 90 percent; standing the solution for 24 hours; and filtering the obtained solution, taking filter residue, washing the obtained product and drying to obtain the spreading hedyotis herb polysaccharide. By adopting the method for preparing the spreading hedyotis herb polysaccharide, the product has high purity and the industrial amplification is easy to be realized.

Description

technical field [0001] The invention relates to a preparation method of Hedyotis diffusa polysaccharide, in particular to a preparation method of extracting Hedyotis diffusa polysaccharide from plants. Background technique [0002] Hedyotis diffusa is the whole plant of Hedyotis diffusa Oldenlandia diffusa (Willd.) Roxb. of the Rubiaceae plant. It is cold in nature, bitter in taste, sweet, and has the effects of clearing away heat and detoxification, diuresis and swelling, antibacterial and anti-inflammatory, etc. It is often used clinically for the treatment of tumors and autoimmune diseases. The polysaccharides in it have anti-tumor, anti-bacterial and anti-inflammatory effects, and improve the body's immunity, and are important active ingredients. [0003] Some preparation methods of Hedyotis diffusa polysaccharides are recorded in the prior art, such as "Research on the Extraction and Purification Process of Hedyotis diffusa Polysaccharides" [Yang Peimin, et al. Qilu Ya...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P31/04A61P29/00A61P37/04
Inventor 朱缨
Owner SUZHOU HEALTH COLLEGE
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