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Combined adjuvant for fish soaked vaccine and its application and use method

A technology of soaking vaccines and combined application, which is applied in the direction of medical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of fish body injury, low work efficiency, and few products, and achieve improved immune effects, Easy to use and safe to use

Inactive Publication Date: 2011-12-07
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish injection vaccines have obvious effect on disease prevention, but injection immunization workload is heavy, work efficiency is low, and it will cause damage to fish
The fish soaking vaccine does not have the above disadvantages, but the simple use of vaccine soaking effect is not good
Currently, there are not many vaccines developed for fish immersion immunization due to the limitation of poor immersion immunization effect.
Domestically developed adjuvants for fish soaked vaccines include hyoscyamine, dextran, and IMS1312. Tests have shown that the immune protection rate after use is not ideal
Most foreign fish-soaked immune adjuvants are secret formulas
Based on the above status quo, the speed of research and development of fish soaked vaccines in my country is in a state of slow development, and restrictions hinder the development of the industry

Method used

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  • Combined adjuvant for fish soaked vaccine and its application and use method
  • Combined adjuvant for fish soaked vaccine and its application and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: application concentration of the present invention is respectively 1mg / L, 0.1mg / L, 60mg / L DTT, VD3, 654-2 combined adjuvant and inactivated Vibrio anguillarum (Vibrio.Anguillarum) vaccine to flounder immersion immunity

[0017] 1 Material:

[0018] (1) Test fish and breeding management: select healthy flounder with a weight of 5g, and raise them temporarily for 1 week before the test. The test seawater is stable at 20-23°C, pH8.1-8.2, salinity 30, and fed with compound bait during the test. , fed twice a day, continuously inflated for 24 hours, running water culture.

[0019] (2) Vaccine preparation: inoculate V. anguillarum into 2216E liquid medium, incubate at 28°C for 24h; centrifuge at 4000rpm at 4°C for 20min to collect the bacteria; place the collected bacteria in sterilized PBS, pH 7.2, to prepare Vibrio suspension of 1.0×108cells / mL, inactivated by 0.5% formaldehyde at 28°C for 48 hours, then stored at 4°C; at the same time, the inactivated bacte...

Embodiment 2

[0030] Embodiment 2: Application concentration of the present invention is respectively 50mg / L, 20mg / L, 1mg / L combined adjuvant of DTT, VD3, 654-2 and inactivated Aeromonas dot-shaped vaccine to tilapia immersion immunization

[0031] 1 Material:

[0032] (1) Test fish and breeding management: choose healthy tilapia with a weight of 3g, and temporarily raise them for 10 days before the test. Feed 2 times, change 30% of the water daily, and inflate continuously for 24 hours.

[0033] (2) Vaccine preparation: Inoculate Aeromonas punctatus into LB liquid medium, culture at 28°C for 24h; centrifuge at 4000rpm for 20min at 4°C, collect the bacteria; place the collected bacteria in sterilized normal saline, In pH 7.2, make a bacterial suspension of 1.0×109cells / mL, inactivate 0.5% formaldehyde at 28°C for 48 hours, then store it at 4°C; Cultivate at a constant temperature of 28°C for 24h and 48h, and confirm that no colony grows.

[0034] (3) Immune adjuvants soaked: DTT, VD3, 65...

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Abstract

A combined adjuvant for soaked vaccines for fish and its application and use method, characterized in that the combined adjuvant is a combination of three adjuvants of dithiothreitol, vitamin D3 and racemic anisodamine, wherein dithiothreose Alcohol, vitamin D3 and racemic anisodamine are used at concentrations of 1-50mg / L, 0.1-20mg / L and 1-60mg / L respectively, and the synergistic effect is achieved in soaking vaccines including seawater and freshwater fish. immune effect. When using, first select fish to be immunized with a body weight of more than 2g per fish and with intact body surface; move the fish to be immunized into the dithiothreitol pretreatment solution with a concentration range of 1-50mg / L and soak for 1-20min, and then Add VD3 with a concentration range of 0.1-20mg / L and the vaccine to soak for 2-20 minutes, then add racemic anisodamine with a concentration range of 1-60mg / L and soak for 5-20 minutes. The invention significantly improves the immune effect of the vaccine, has simple and safe use method, low cost, does not need additional facilities and procedures, has no influence on the survival rate and growth speed of fish, and is also harmless to the environment and operators.

Description

technical field [0001] The invention relates to the immune prevention and control technology of marine biological diseases, in particular to a combination adjuvant for fish soaked vaccine and its application and use method. Background technique [0002] The output of farmed fish in my country ranks first in the world, but the research and development of fish disease prevention and control technology is slow, and the developed fish disease prevention and control products cannot meet the needs of production, especially in the development and application of fish vaccines, which are currently available for fishery production. Not many products to choose to use. Therefore, the treatment of fish bacterial diseases in aquaculture relies too much on feeding antibiotics. As a result, long-term application of antibiotics will not only reduce the quality of aquatic products, but also lead to enhanced bacterial resistance, making it more difficult for future disease control. Foreign cou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39
Inventor 王秀华黄倢王玉娟赵伟伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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