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Method for conveniently and fast observing stem tip flower bud differentiation of tobacco plant

A flower bud differentiation and stem tip technology, applied in the field of plant developmental biology, can solve the problems of no public reports, high cost of scanning electron microscope method, complicated operation of paraffin section method, etc., and achieve the effect of simple operation and low cost

Inactive Publication Date: 2010-07-28
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the paraffin section method is cumbersome to operate, and the cost of scanning electron microscopy is relatively high.
[0003] Through literature search, there is no public report identical with the present invention

Method used

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  • Method for conveniently and fast observing stem tip flower bud differentiation of tobacco plant
  • Method for conveniently and fast observing stem tip flower bud differentiation of tobacco plant
  • Method for conveniently and fast observing stem tip flower bud differentiation of tobacco plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Adopt the conventional floating seedling raising method to raise seedlings, transplant the tobacco seedlings into flowerpots after they become seedlings, take samples of 1-2 plants 20 days after transplanting, cut off the stem tips from the stem tip 5 cm downwards, and put the stem tips in the pot. Put it into an ordinary medical syringe (above 15mL), push the plunger to the bottom, block the front of the needle with your thumb, and pull the plunger to the top for 1, 2 or 3 minutes, repeat 3 or 4 times. The samples were fixed in FAA fixative to be tested. The proportioning ratio of FAA fixative solution (100mL) is: 38% formaldehyde 5.0mL, acetic acid 5.0mL, 75% ethanol 90.0mL. After 24 hours, take the sample out and insert a thin needle at the base of the stem. The other end of the needle is inserted on a plastic foam with a length, width and height of 5×5×1cm, and then put the plastic foam on the OLYMPUS SZX12 stereo microscope On the stage, move the plastic foam and ...

Embodiment 2

[0025] The selected varieties are coated species of Yunyan 85, Yunyan 87, Hongda, K326, Yunyan 97 and Yunyan 201, and one-stage floating seedlings are adopted. When the tobacco seedlings grew to 7 true leaves, low temperature induction treatment was started, which were divided into control and low humidity treatment. The control tobacco seedlings were managed as normal seedlings in the shed, and the low temperature induction was carried out in an artificial climate box, with a day and night temperature of 13.0°C / 6.0°C , light time 8h / d, light intensity 1 000-1 500lx. After 10 days of low-temperature induction, 15 low-temperature-treated tobacco seedlings and 15 control tobacco seedlings were transplanted for pot experiment. Observe according to embodiment 1 after transplanting 20d, and observation result is shown in Table 1. It can be seen from Table 1 that the flower bud differentiation time of different varieties is different, and the sensitivity of flower bud early differe...

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Abstract

The invention relates to a method for conveniently and fast observing stem tip flower bud differentiation of tobacco plant, belonging to the technical field of developmental biology of plants. The method comprises the following steps: a. taking a sample with a blade at the position which is 5 centimetres downward from the stem tip; b. after sampling, immediately placing the stem tip in an ordinary injector for medical use, which has a volume of more than 15mL, pushing a pushing rod of the injector to the bottom, using a thumb to block the front part of a needle tube and forcefully pulling up the pushing rod to the top of the injector; retaining the position for 1-3min and repeating the process for 3-4 times; c. fixing the stem tip sample in a stationery liquid for 24h for testing with components having the following proportioning: 5.0mL of formaldehyde with a concentration of 38%, 5.0mL of acetic acid, 90.0mL of alcohol with a concentration of 75%; d. using a stereomicroscope to observe and take photos of the sample. The method has the advantages of low cost, simple and convenient operation, fast observation and acquisition of digitalized pictures with clear images, thus being of great guiding significance for experimental studies.

Description

Technical field: [0001] The invention relates to a method for conveniently observing the differentiation of stem tip flower buds of tobacco plants, which belongs to the technical field of plant development biology. Background technique: [0002] Flower bud differentiation is a sign of a plant's transition from vegetative growth to reproductive growth. The morning and evening of flue-cured tobacco flower bud differentiation not only affect the number of effective leaves and the yield of tobacco leaves, but also have a significant impact on the quality of tobacco leaves. Chen Ruitai [1] Yue Caipeng [2] Using paraffin section method to study and report the morphological changes of stem end tissue in the process of flower bud differentiation, Liu Xiuli [3] studied the process and characteristics of flower bud development by scanning electron microscopy, and analyzed the relationship between inflorescence construction and leaf type. However, the paraffin section method is com...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/06G01N1/28
Inventor 晋艳段玉琪杨宇虹金磊周冀衡黄成江徐照丽
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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