Amino acid sequence with characteristic of bonding histone deacetylase 1 and expression vector thereof

Abstract

The invention discloses an amino acid sequence with a characteristic of bonding histone deacetylase 1 and an expression vector thereof, which belongs to the field of biotechnology. The amino acid is conservative in sequential evolution and has the characteristic of special bonding histone deacetylase 1. After an expression vector pcDNA3-FATS constructed by the amino acid sequence undergoes cell transfection, the expression level of periodical inhibitory protein p21 of a specific induced cell is improved; and the combination of the bonding histone deacetylase 1 and the p21 is restrained to increase the acetylation modification of the p21 protein and restrain degradation of the p21 protein. The amino acid sequence and the expression vector thereof prove the anti-cancer effect of a small molecular compound of inhibition histone deacetylase applied to a clinical experiment and provide a natural simulation target spot for the research and development of a new-generation more specific anti-HDAC1 chemical medicament.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an amino acid sequence with the characteristic of binding histone deacetylase 1 and an expression vector thereof. Background technique [0002] The cell cycle negative regulatory protein p21 mainly regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases, and is regulated by the p53 tumor suppressor gene at the transcriptional level. The lack of p21 will lead to cell cycle G after DNA damage. 2 The / M node function is severely disrupted, and increasing the protein expression level of p21 leads to cell cycle arrest at G 1 Expect. A class of anticancer drugs (histone deacetylase inhibitors) currently entering clinical phase II trials such as SAHA (suberoylanilide hydroxamicacid, N-hydroxy-N′-phenylsuberamide; vorinostat) and a series of TSA (trichostatin A, trichostatin A) derivatives, etc., the common mechanism of action is to induce the increase of p21 protei...

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Problems solved by technology

[0002] The cell cycle negative regulatory protein p21 mainly regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases, and is regulated by the p53 tumor suppressor gene at the transcriptional level. The lack of p21 will lead to cell cycle G after DNA damage. 2 The / M node function is severely disrupted, and increasing the protein expression level of p21 leads to cell cycle arrest at G 1 Expect

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