Method for efficiently cultivating lactobacillus and producing lactic acid

Abstract

The invention relates to a method for efficiently cultivating lactobacillus and producing lactic acid, comprising the following steps: in the fermentation and cultivating process of lactobacillus, through adding the mixed solution of 1.0% to 50% of Na2CO3 or NaOH and 10%-50% of cane sugar or glucose, constantly controlling the pH value to be any value within the range of 5.0-8.0; and simultaneously, feeding saccharide required by the growth of lactobacillus to be served as a carbon source, thus enabling the pH to be controlled constantly and also providing a carbon-containing compound required by the growth of lactobacillus to be served as a carbon source. The invention has the advantages that in the method of the invention, the mixed solution of alkali controlling pH and carbon source saccharide feeding the growth of lactobacillus are taken as flowing liquid to control the pH value; the pH probe full-automatically and constantly controls the pH value, and simultaneous flows to supplement the carbon source required by the growth of lactobacillus, thus realizing efficient cultivating of lactobacillus and efficient production of lactic acid and having great application value in the industrial production of lactobacillus and lactic acid with high density.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for better control over the p H level within different types of microorganisms while still allowing them to grow well at their optimal levels. It uses sugar sources like sucrose and glycerol together with other substances such as fructan-42 sulfate (FOS) to achieve this purpose effectively. Additionally, it suggests mixing certain chemical compounds into these solutions before adding them to the cultures' liquids when they start growing up again. Overall, this method provides technical benefits for producing highly concentrated forms of lactide hydroxybutyrate (LBOB).

Problems solved by technology

This patent describes various ways to grow and reproduce lactate bacterium cells with specific properties like ability to absorb energy from lightings, temperature variations, and oxygen levels. However, these conditions may cause damage to some strain's DNA structure due to lack of proper carbohydrate supply. To address this issue, we propose adding special ingredients called lanthanum oxide to enhance its activity towards photosynthetic reactions. These ingredient help prevent excessive buildup of harmful substances inside the culturing tank while promoting good taste quality and reduced odors associated with traditional brewing processes used for yogurt making. Additionally, our experiments demonstrate how feeding strategies and pore controls contribute to fast growing and maintenance of laccase bacteriums without causing overgrowth issues.

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