Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
An amino acid and reagent technology, which is applied in the field of amino acid concentration determination, amino acid diagnosis/determination reagents, and can solve the problems of high cost of instruments and equipment, poor specificity, and complicated operation methods.
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Embodiment 1
[0046] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0048] Stabilizer 500mmol / L
[0049] Coenzyme 3mmol / L
[0050] Amino acid oxidase 10000U / L
[0051] Pyruvate oxidase 12000U / L
[0052] Lovastatin Nine Ketone Synthase 12000U / L
[0053] Sodium bicarbonate 9mmol / L
[0054] Acetyl-CoA 6mmol / L
[0055] Dihydromonascus L 6mmol / L
[0056] S-adenosyl-homocysteine 6mmol / L
[0057] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0058] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the amino acid sample to be tested is 1 / 25, the reaction The direction is positive reaction (...
Embodiment 2
[0061] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0062] Reagent 1
[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0064] Stabilizer 50mmol / L
[0065] Coenzyme 3mmol / L
[0066] Sodium bicarbonate 9mmol / L
[0067] Acetyl-CoA 6mmol / L
[0068] Dihydromonascus L 6mmol / L
[0069] S-adenosyl-homocysteine 6mmol / L
[0070] Reagent 2
[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0072] Stabilizer 50mmol / L
[0073] Amino acid oxidase 10000U / L
[0074] Pyruvate oxidase 12000U / L
[0075] Lovastatin Nine Ketone Synthase 12000U / L
[0076] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0077] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the vol...
Embodiment 3
[0080] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:
[0081] Reagent 1
[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0083] Stabilizer 50mmol / L
[0084] Coenzyme 3mmol / L
[0085] Sodium bicarbonate 9mmol / L
[0086] Acetyl-CoA 6mmol / L
[0087] Dihydromonascus L 6mmol / L
[0088] S-adenosyl-homocysteine 6mmol / L
[0089] Reagent 2
[0090] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0091] Stabilizer 500mmol / L
[0092] Pyruvate oxidase 12000U / L
[0093] Lovastatin Nine Ketone Synthase 12000U / L
[0094] Reagent 3
[0095] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0096] Stabilizer 500mmol / L
[0097] Amino acid oxidase 10000U / L
[0098] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0099] When measuring amino acid concentration, set on the...
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