Fermentation technology of 3Ec nano enzyme
A technology of coenzyme and laccase, which is applied in the field of enzyme composition to promote coal combustion efficiency and reduce the generation of nitrogen and sulfur compounds, and can solve problems such as acid rain and environmental pollution
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[0029] 1. Preparation of the enzyme composition of the present invention
[0030] Take 5ml of laccase isoenzyme (CAS NO.80498-15-3) with a concentration of 1ppm and 50g of ammonium acetate, add it to 50L of clean water and evenly stir, and store at 4°C-15°C before use.
[0031] 2. Treatment of coal with the enzyme composition of the present invention
[0032] First, the coal is ground into pulverized coal, 80% of which can pass through a 200-mesh sieve, and the pulverized coal is roasted so that its moisture content is less than 17%. The enzyme composition prepared in Example 1 was evenly sprayed on the dried pulverized coal, and the pulverized coal was evenly stirred at 9, 12, and 17 o'clock, respectively. The sprayed pulverized coal was allowed to stand for 72 hours under the condition of avoiding sunlight, and the combustion experiment was carried out after standing for 72 hours. The control group was sprayed with water.
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