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Method for preparing living paratyphoid vaccine for piglets and product thereof

A technology for piglet paratyphoid and live vaccine, which can be applied to medical preparations containing active ingredients, pharmaceutical formulations, antibacterial drugs, etc., and can solve the problems of low yield, difficult production, and low bacterial count

Active Publication Date: 2010-06-16
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is to overcome the problems of low bacterial count, high freeze-drying mortality rate, large production difficulty, low output and high cost in the existing production method of piglet paratyphoid live vaccine, and provide a new piglet vaccine A production method of paratyphoid live vaccine, which can shorten the bacterial culture time, significantly increase the number of cultured bacteria, and greatly reduce the freeze-drying mortality rate

Method used

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  • Method for preparing living paratyphoid vaccine for piglets and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of primary strains:

[0029] Dilute the strain of Salmonella choleraesuis (purchased from the China Veterinary Drug Control Institute) with ordinary broth or culture and propagate it in ordinary broth, inoculate on ordinary agar plate, culture at 37°C for 18-20h, and select colonies that are round, with neat edges and protruding , 5-10 translucent, slightly moist smooth medium-sized colonies, mixed and inoculated on several branches of ordinary agar slant, cultured at 37°C for 24 hours, tested purely, and used 1 / 500 acridine yellow solution to do the slide agglutination test to check that it was qualified. As a first-class strain, it should be stored at 2-8°C, and it should not exceed 2 months. It can be transplanted 1-2 times during this period, and it can be subcultured on the culture medium for no more than 5 generations.

[0030] 2. Preparation of secondary strains:

[0031] Hook the first-grade strains and inoculate them into ordinary broth agar fla...

Embodiment 2

[0035] 1. Preparation of primary strains:

[0036]Dilute the strains of Salmonella choleraesuis (please give the commercial purchase channel of the strains of Salmonella choleraesuis) with ordinary broth or after culture and propagation in ordinary broth, inoculate the common agar plate, culture at 37°C for 18-20h, select the colony as a circle 5-10 medium-sized colonies with neat shape, neat edges, protruding, translucent, and slightly moist smooth, mixed and inoculated on several branches of ordinary agar slant, cultured at 37°C for 24 hours, tested purely, and made glass with 1 / 500 acridine yellow solution The sheet agglutination test is qualified, as a first-class strain, it should be stored at 2-8°C, and it should not exceed 2 months. It can be transplanted 1-2 times during this time, and it can be subcultured on the medium for no more than 5 generations.

[0037] 2. Preparation of secondary strains:

[0038] Hook the first-grade strains and inoculate them in common brot...

Embodiment 3

[0042] 1. Preparation of primary strains:

[0043] Dilute the strains of Salmonella choleraesuis (please give the commercial purchase channel of the strains of Salmonella choleraesuis) with ordinary broth or after culture and propagation in ordinary broth, inoculate the common agar plate, culture at 37°C for 18-20h, select the colony as a circle 5-10 medium-sized colonies with neat shape, neat edges, protruding, translucent, and slightly moist smooth, mixed and inoculated on several branches of ordinary agar slant, cultured at 37°C for 24 hours, tested purely, and made glass with 1 / 500 acridine yellow solution The sheet agglutination test is qualified, as a first-class strain, it should be stored at 2-8°C, and it should not exceed 2 months. It can be transplanted 1-2 times during this time, and it can be subcultured on the medium for no more than 5 generations.

[0044] 2. Preparation of secondary strains:

[0045] Hook the first-grade strains and inoculate them into ordinary...

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Abstract

The invention discloses a method for producing living paratyphoid vaccine for piglets and a product thereof. The method comprises the following steps: (1) preparing primary strains; (2) preparing secondary strains; and (3) culturing bacterial liquid: inoculating the secondary strains into a culture medium for culturing, after culturing, immediately adding a stabilizing agent which is sterilized and preheated to 37 DEG C to ensure that the vaccine contains 1.5 percent of gelatin and 5 percent of sucrose, and mixing uniformly to obtain the bacterial liquid, wherein the culture medium in the step (3) consists of a common broth medium and a synthetic medium in the volume ratio of 2:1. In the living paratyphoid vaccine for piglets produced by the method, the cultured bacteria count is over 50 billion per milliliter; the maximum cultured bacteria count is 58 billion per milliliter; and the freeze-drying death rate is reduced to 20-40 percent. The method for producing the living paratyphoid vaccine for the piglets has the advantages of high bacterial count of the living vaccine, low freeze-drying death rate, easy production, high yield, low production cost, and the like.

Description

technical field [0001] The invention relates to a preparation method of a live animal vaccine, in particular to a preparation method of a live piglet paratyphoid vaccine and a product prepared by the method, and belongs to the field of live paratyphoid vaccines. Background technique [0002] Piglet paratyphoid is an acute, subacute or chronic infectious disease caused by Salmonella choleraesuis. It mainly occurs in piglets aged 2-4 months, and is extremely harmful. The acute case mainly manifests as sepsis, and the symptoms are sudden rise in body temperature, lack of energy, and lack of food. Later, there are diarrhea, dyspnea, ear root, chest and abdomen The skin has purple spots, and sometimes dies within 24 hours after the onset of symptoms, and the case fatality rate is very high. Subacute and chronic symptoms manifest as weight loss, diarrhea and enteritis, and the disease is often delayed for 2-3 weeks or longer, and finally becomes extremely emaciated and dies of ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/112A61P31/04
Inventor 刘洪斌邬立权张继东付丽杰王静杨万秋左颖李尊江
Owner 哈药集团生物疫苗有限公司
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